DLD 抗体 (HRP)
-
- 抗原 See all DLD 抗体
- DLD (Dihydrolipoamide Dehydrogenase (DLD))
-
适用
- Pig
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This DLD antibody is conjugated to HRP
-
应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- Supplier Product No.
- 200-4360-0100
- Supplier
- Rockland
- 原理
- Lipoamide Dehydrogenase Antibody Peroxidase Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Lipoamide Dehydrogenase [Porcine Heart].
- 产品特性
- Synonyms: rabbit anti-Lipoamide Dehydrogenase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Lipoamide Dehydrogenase Antibody, Dihydrolipoyl dehydrogenase mitochondrial, Dihydrolipoamide dehydrogenase, Lipoamide Dehydrogenase
- 纯化方法
- Lipoamide Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
-
Immunogen: Lipoamide Dehydrogenase [Porcine Heart]
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product DLD Primary Antibody
-
anti-Dihydrolipoamide Dehydrogenase (DLD) (AA 45-271) antibody
DLD 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) antibodyDLD 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) antibodyDLD 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) antibodyKO Validated DLD 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) (AA 1-509) antibodyDLD 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3C1 unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) (AA 36-300) antibodyDLD 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) (AA 238-466) antibodyDLD 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) antibodyDLD 适用: 人, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) antibodyDLD 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IF, FACS 宿主: 小鼠 Monoclonal 5G7 unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) (C-Term) antibodyDLD 适用: 人, 小鼠, 大鼠 WB, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
-
Application Note: Anti-Lipoamide Dehydrogenase Peroxidase Conjugated Antibody has been tested by ELISA and Western blot and is assayed against 1.0 μg of Lipoamide Dehydrogenase [Porcine Heart] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
-
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! - 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
-
- 抗原
- DLD (Dihydrolipoamide Dehydrogenase (DLD))
- 别名
- DLD LAD (DLD 产品)
- 背景
- Background: Lipoamide Dehydrogenase encodes the L protein of the mitochondrial glycine cleavage system. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency.
- 基因ID
- 397129, 118675
- UniProt
- P09623
- 途径
- Ribonucleoside Biosynthetic Process, Cell RedoxHomeostasis
-
