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Catalase 抗体 (HRP)

The 兔 多克隆 anti-Catalase antibody is suitable to detect Catalase in samples from Cow. It has been validated for WB 和 ELISA.
Rockland
产品编号 ABIN5596704
Supplier Product No.: 200-4351-0100
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Catalase 抗体 (HRP) (ABIN5596704)

抗原

See all Catalase (CAT) 抗体
Catalase (CAT)

适用

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Cow

宿主

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克隆类型

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多克隆

标记

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This Catalase antibody is conjugated to HRP

应用范围

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Western Blotting (WB), ELISA
  • Supplier Product No.

    200-4351-0100

    Supplier

    Rockland

    原理

    Catalase Antibody Peroxidase Conjugated

    交叉反应 (详细)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Catalase [Bovine Liver].

    产品特性

    Synonyms: rabbit anti-Catalase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Catalase Antibody, Cas 1 antibody, CAT antibody, Cs1 antibody, MGC138422 antibody, MGC138424 antibody

    纯化方法

    Catalase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

    免疫原

    Immunogen: Catalase [Bovine Liver]

    Immunogen Type: Native Protein

    亚型

    IgG
  • 应用备注

    Application Note: Anti-Catalase Peroxidase Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Catalase [Bovine Liver] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500- 1:2,500

    ELISA Dilution: 1:2,000 - 1:10,000

    Other: User Optimized

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度

    10.0 mg/mL

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

    储存液

    Gentamicin sulfate

    注意事项

    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

    有效期

    12 months
  • 抗原

    Catalase (CAT)

    别名

    CAT

    背景

    Background: Catalase is found in nearly all living organisms exposed to oxygen. It catalyzes the decomposition of hydrogen peroxide to water and oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species.

    基因ID

    531682

    UniProt

    P00432

    途径

    Cellular Glucan Metabolic Process, Cell RedoxHomeostasis, Photoperiodism
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