CRISPR-Cas9 (C-Term) 抗体 (Biotin)
Quick Overview for CRISPR-Cas9 (C-Term) 抗体 (Biotin) (ABIN5596702)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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Supplier Product No.
- 600-406-gk0
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Supplier
- Rockland
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原理
- Cas 9 Antibody Biotin Conjugated
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交叉反应 （详细）
- Anti-Cas9 antibody is directed against Streptococcus pyogenes Cas-9.
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产品特性
- Synonyms: rabbit anti-Cas9 antibody biotin conjugation, biotin conjugated rabbit anti-Cas9 antibody, Cas-9, Cas 9, CRISPR, CRISPR-associated endonuclease Cas9/Csn1, SpCas9, SpyCas9, csn1, Cas-9 Antibody
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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免疫原
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Immunogen: Cas-9 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of Streptococcus pyogenes Cas-9 protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-CRISPR-Cas9 (AA 1-462) antibody
适用: Staphylococcus aureus WB 宿主: 小鼠 Monoclonal unconjugated Recombinant Antibody
anti-CRISPR-Cas9 antibody适用: Staphylococcus aureus WB, IF 宿主: 兔 Polyclonal unconjugated
anti-CRISPR-Cas9 antibody适用: Streptococcus pyogenes WB, IF, IP, ChIP 宿主: 小鼠 Monoclonal 8C1-F10 unconjugated
anti-CRISPR-Cas9 (C-Term) antibody适用: Streptococcus pyogenes WB, IF, ELISA, FM, MA 宿主: 兔 Polyclonal unconjugated
anti-CRISPR-Cas9 antibody适用: 人 WB 宿主: 小鼠 Monoclonal 1E8 unconjugated
anti-CRISPR-Cas9 (C-Term) antibody (DyLight 680)WB, ELISA, FM 宿主: 兔 Polyclonal DyLight 680
anti-CRISPR-Cas9 (C-Term) antibody (DyLight 549)WB, ELISA, FM 宿主: 兔 Polyclonal DyLight 549
anti-CRISPR-Cas9 (C-Term) antibody (DyLight 488)WB, ELISA, FM 宿主: 兔 Polyclonal DyLight 488
anti-CRISPR-Cas9 (C-Term) antibody (DyLight 800)WB, ELISA, FM 宿主: 兔 Polyclonal DyLight 800
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应用备注
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Application Note: Cas9 Biotin Conjugated Antibody has been tested for use in ELISA and suitable for Immunofluorescence and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band ~158 kDa in size corresponding to Cas-9 protein by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1 μg/mL
ELISA Dilution: 1:25,000-1:30,000
IF Microscopy Dilution: 1 μg/mL
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- CRISPR-Cas9
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别名
- cas9
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背景
- Background: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR Associated (Cas) system was discovered in bacteria as a defense against foreign DNA, even being believed to act as a sort of prokaryotic immune system. The Cas9 protein is becoming a useful tool in the field of genomic editing for its ability to induce site-directed double strand breaks in DNA. It can cleave almost any sequence complementary to the guide RNA and has recently been used for modifying the genome of human embryos for the first time.
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基因ID
- 901176
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NCBI登录号
- NP_269215
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UniProt
- Q99ZW2
抗原
-
