APOE 抗体
Quick Overview for APOE 抗体 (ABIN5596681)
抗原
See all APOE 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-101-197
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Supplier
- Rockland
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原理
- APOLIPOPROTEIN E Antibody
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交叉反应 (详细)
- Typically less than 1 % cross-reactivity against other types of apoLipoprotein was detected by ELISA against purified standards.
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产品特性
- Synonyms: goat anti-Apolipoprotein E3 antibody, Alzheimer disease 2 antibody, Apo-E antibody, Apo E antibody, ApoE antibody, APOEA antibody, Apolipoprotein-E antibody, Apoprotein antibody
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纯化方法
- Anti-Apolipoprotein E has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Apolipoprotein Type E was isolated from human plasma by density gradient centrifugation followed by HPLC purification.
Immunogen Type: Native Protein
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亚型
- IgG
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应用备注
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Immunohistochemistry Dilution: 1:50 - 1:200
Application Note: Apolipoprotein E antibodies have been tested by western blot and used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis.
Western Blot Dilution: 1:500 - 1:2,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:4,000 - 1:8,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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Spontaneous severe hypercholesterolemia and atherosclerosis lesions in rabbits with deficiency of low-density lipoprotein receptor (LDLR) on exon 7." in: EBioMedicine, Vol. 36, pp. 29-38, (2019) (PubMed).
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Spontaneous severe hypercholesterolemia and atherosclerosis lesions in rabbits with deficiency of low-density lipoprotein receptor (LDLR) on exon 7." in: EBioMedicine, Vol. 36, pp. 29-38, (2019) (PubMed).
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- APOE (Apolipoprotein E (APOE))
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别名
- APOE
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背景
- Background: Anti Apolipoprotein E Antibody recognizes the gene product of APOE. Apolipoprotein E is found in the chylomicron and IDLs that binds to a specific receptor on liver cells and peripheral cells. Apolipoprotein E is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This antibody is suitable for cardiovascular research.
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基因ID
- 348
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NCBI登录号
- NP_000032
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UniProt
- P02649
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途径
- Regulation of Cell Size, Lipid Metabolism
抗原
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