Recombinant TET1 抗体 (AA 1682-1914) (Sortase,His tag,AVI tag)
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Quick Overview for Recombinant TET1 抗体 (AA 1682-1914) (Sortase,His tag,AVI tag) (ABIN5563986)
抗原
See all TET1 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1682-1914
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原理
- AbFlex TET1 antibody (rAb)
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品牌
- AbFlex®
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产品特性
- AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.
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纯化方法
- Protein A Chromatography
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免疫原
- This antibody was raised against a recombinant protein comprising amino acids 1682-1914 of mouse TET1 (Ten-Eleven Translocation-1, methylcytosine dioxygenase).
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亚型
- IgG2a
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anti-Tet Methylcytosine Dioxygenase 1 (TET1) (AA 2038-2136) antibody
TET1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4F4 unconjugated
anti-Tet Methylcytosine Dioxygenase 1 (TET1) (AA 194-417) antibodyTET1 适用: 人, 猴 WB, ELISA, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Tet Methylcytosine Dioxygenase 1 (TET1) (C-Term) antibodyTET1 适用: 人, 小鼠 WB, IHC, ChIP-seq, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Tet Methylcytosine Dioxygenase 1 (TET1) (AA 1845-1879), (C-Term) antibodyTET1 适用: 小鼠 WB 宿主: 兔 Polyclonal RB52496 unconjugated
anti-Tet Methylcytosine Dioxygenase 1 (TET1) (AA 1700-1800) antibodyTET1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- ChIP-Seq: 4 µL per ChIP AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 61741.
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说明
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This recombinant antibody has been generated using defined DNA sequences to achieve high specificity and high reproducibility. The unique advantages of this antibody are its flexible labeling and purification options as it contains a sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner. Every antibody also contains a 6xHis Tag, which can be used with nickel-based purification systems, and a Biotinylation Tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- -20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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- TET1 (Tet Methylcytosine Dioxygenase 1 (TET1))
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别名
- TET1
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背景
- AbFlex TET1 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. TET1(Ten-Eleven Translocation-1, methylcytosine dioxygenase) is a protein that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) which can lead to cytosine demethylation by either further oxidation via TET proteins to 5-formylcytosine (5fC) and 5-carboxycytosine (5caC) or deamination into 5-hydroxymethyluracil (5hmU) and then subsequent replacement by unmethylated cytosine by the base excision repair system. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. TET1 preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and polycomb-repressed genes. By controlling the levels of 5mC and 5hmC at gene promoters, it may regulate the gene expression silencing induced by cytosine methylation. May have a dual function by also repressing the expression of a subset of genes through recruitment of transcriptional repressors to promoters.
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分子量
- 215 kDa
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NCBI登录号
- NP_001240786
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途径
- Stem Cell Maintenance, Warburg Effect
抗原
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