Recombinant H2AFV 抗体 (Sortase,His tag,AVI tag)
Our Local Distributor
北京 101111
Quick Overview for Recombinant H2AFV 抗体 (Sortase,His tag,AVI tag) (ABIN5563978)
抗原
See all H2AFV 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
-
-
原理
- AbFlex AM-Tag antibody (rAb)
-
品牌
- AbFlex®
-
特异性
- Drosophila
-
产品特性
- AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.
-
纯化方法
- Protein A Chromatography
-
免疫原
- This antibody was raised against Active Motif's unique AM-Tag sequence for use with Tag-ChIP-IT (Cat No. 53022) analysis.
-
亚型
- IgG2a
-
-
anti-H2A Histone Family, Member V (H2AFV) (AA 1-128) antibody
H2AFV 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-H2A Histone Family, Member V (H2AFV) (AA 1-128) antibodyH2AFV 适用: 人 ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2F9 unconjugated
anti-H2A Histone Family, Member V (H2AFV) (C-Term) antibodyH2AFV 适用: Drosophila melanogaster WB, ChIP 宿主: 小鼠 Monoclonal 10E9-D1 unconjugated
anti-H2A Histone Family, Member V (H2AFV) (AA 3-128) antibodyH2AFV 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-H2A Histone Family, Member V (H2AFV) (AA 1-129) antibodyH2AFV 适用: 人 ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal 2F9 unconjugated
anti-H2A Histone Family, Member V (H2AFV) (N-Term) antibodyH2AFV 适用: 人, Drosophila melanogaster, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, 绵羊, 斑马鱼, 小鸡, 猴, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- ChIP: 10 μg per ChIP WB: 0.5 - 2 μg/mL dilution Bead-based ELISA: 7 - 560 ng/mL
-
说明
-
This recombinant antibody has been generated using defined DNA sequences to achieve high specificity and high reproducibility. The unique advantages of this antibody are its flexible labeling and purification options as it contains a sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner. Every antibody also contains a 6xHis Tag, which can be used with nickel-based purification systems, and a Biotinylation Tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
- Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 40 % glycerol and 0.035 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
-
储存条件
- -20 °C
-
储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
-
有效期
- 24 months
-
-
- H2AFV (H2A Histone Family, Member V (H2AFV))
-
别名
- Histone H2Av
-
背景
- AbFlex AM-Tag antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. The AM-Tag antibody is specifically designed for use with unique AM-Tag sequence in combination with the Tag-ChIP-IT Kit (Catalog No. 53022). Simply clone your protein of interest into pAM_1C Empty Vector (Catalog No. 53023), or add the AM-Tag sequence to the C-terminus of your protein expression vector. Following transfection, cell lysates are prepared and screened by Western blot for expression of the AM-Tag fusion protein using the AM-Tag antibody. Optimization may be required to identify the optimal conditions for transfection and protein expression. Once transfection conditions have been optimized, the Tag-ChIP-IT Kit can be used to isolate chromatin and perform ChIP. The AM-Tag has minimal cross reactivity with mammalian samples to ensure low background signal. The tag is also unstructured, which allows the tag to protrude from the protein of interest for maximum exposure during immunoprecipitation. This increases the enrichment efficiency of low abundance transcription factors for more reliable and consistent ChIP results. Use of the AM-Tag for chromatin immunoprecipitation avoids the need for protein-specific ChIP-validated antibodies. It also enables the analysis of sequence variants, mutations and truncations on gene regulation.
-
分子量
- 15 kDa
-
NCBI登录号
- NP_524519
抗原
-
