Recombinant EED 抗体
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- 抗原 See all EED 抗体
- EED (Embryonic Ectoderm Development (EED))
- 抗体类型
- Recombinant Antibody
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This EED antibody is un-conjugated
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应用范围
- ELISA, ChIP DNA-Sequencing (ChIP-seq), Chromatin Immunoprecipitation (ChIP)
- 品牌
- AbFlex®
- 产品特性
- AbFlex™ EED antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. EED (extraembryonic ectoderm) is a component of the Polycomb group (PcG) multiprotein complex PRC2. The PRC2 complex also contains EZH2 and SUZ12 and is recruited to Polycomb response elements and results in the methylation of histone H3 and lysine 27 and the silencing of gene expression. PRC2 initiates repression of the Hox genes during development and is involved in the maintenance of stem cell pluripotency. EED is a member of the superfamily of WD-40 repeat protein family and is required for PRC2-mediated H3K27 methylation. The PRC2 complex may also recruit platform DNMTs, thereby linking two systems of epigenetic repression. Chromatin IP Assays This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with AbFlex™ EED antibody (rAb). Bridging Antibody to Improve ChIP, MeDIP and IP Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use AbFlex™ EED antibody (rAb).
- 纯化方法
- Ni-NTA
- 亚型
- IgG2a
- Top Product
- Discover our top product EED Primary Antibody
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anti-Embryonic Ectoderm Development (EED) (N-Term) antibody
EED 适用: 人, 小鼠, 大鼠, 犬, Cow, 斑马鱼, 豚鼠, 马, 兔 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Embryonic Ectoderm Development (EED) (AA 1-100) antibodyEED 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Embryonic Ectoderm Development (EED) (AA 342-441) antibodyEED 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3B12 unconjugated
anti-Embryonic Ectoderm Development (EED) (AA 142-441) antibodyEED 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Embryonic Ectoderm Development (EED) (AA 1-300) antibodyEED 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Embryonic Ectoderm Development (EED) (AA 269-303) antibodyEED 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB51180 unconjugated
anti-Embryonic Ectoderm Development (EED) (AA 21-70) antibodyEED 适用: 人, 小鼠, 大鼠, 非洲爪蟾, 犬, Cow, 斑马鱼, 豚鼠, 马, 兔, Bat, 小鸡, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Embryonic Ectoderm Development (EED) (AA 1-441) antibodyEED 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Embryonic Ectoderm Development (EED) (AA 1-300) antibodyEED 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Embryonic Ectoderm Development (EED) (AA 40-74), (N-Term) antibodyEED 适用: 小鼠 WB 宿主: 兔 Polyclonal RB51500 unconjugated
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- 应用备注
- ChIP-Seq: 4 μg per ChIP Bead-based ELISA: 4 - 26 ng/mL
- 说明
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AbFlex® Recombinant Antibodies defined antibodies for highly specific, reproducible performance.
AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. The unique advantages of the AbFlex® antibody are its flexible labeling and purification options. Each AbFlex® antibody contains a Sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner using our Sortag-IT Labeling Kits. Every antibody also contains a 6xHis tag, which can be used with nickel-based purification systems, and an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.
AbFlex® antibodies are specifically labeled at the end of the constant region of the heavy chain to avoid interference with antigen recognition and functionality. This is important as it ensures the labeling process maintains the integrity of the antibody so signal is not diminished as a result of non-functional antibodies. In contrast, commonly used chemical labeling methods add labels to the antibody in a random fashion. The randomness of this process has a high potential to block the antigen-binding site and render the antibody ineffective. Chemical labeling can also deposit labels on the Fc region of the antibody which has the potential to obstruct interactions with protein A. - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 35 mM NaOAc, 0.035 % sodium azide, 30 % glycerol
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
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- 抗原
- EED (Embryonic Ectoderm Development (EED))
- 别名
- EED (EED 产品)
- 别名
- Xeed antibody, heed antibody, wait1 antibody, EED antibody, HEED antibody, WAIT1 antibody, zgc:112509 antibody, Xeed-B antibody, eed antibody, psXEED antibody, ENSMUSG00000039373 antibody, l(7)5Rn antibody, l7Rn5 antibody, lusk antibody, Xeed-A antibody, embryonic ectoderm development antibody, embryonic ectoderm development S homeolog antibody, microRNA 6755 antibody, embryonic ectoderm development L homeolog antibody, eed antibody, EED antibody, eed.S antibody, MIR6755 antibody, Eed antibody, eed.L antibody
- 分子量
- 57 kDa
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