Quick Overview for CRISPR-Cas9 抗体 (ABIN5557542)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
交叉反应
- 人
-
纯化方法
- Purified by Protein A.
-
免疫原
- Recombinant fragment corresponding to Streptococcus pyogenes CRISPR-Cas9 (100-200aa)
-
亚型
- IgM
-
-
anti-CRISPR-Cas9 (AA 1-462) antibody
适用: Staphylococcus aureus WB 宿主: 小鼠 Monoclonal unconjugated Recombinant Antibody
anti-CRISPR-Cas9 antibody适用: Staphylococcus aureus WB, IF 宿主: 兔 Polyclonal unconjugated
anti-CRISPR-Cas9 antibody适用: Streptococcus pyogenes WB, IF, IP, ChIP 宿主: 小鼠 Monoclonal 8C1-F10 unconjugated
anti-CRISPR-Cas9 (C-Term) antibody适用: Streptococcus pyogenes WB, IF, ELISA, FM, MA 宿主: 兔 Polyclonal unconjugated
anti-CRISPR-Cas9 (C-Term) antibody (DyLight 680)WB, ELISA, FM 宿主: 兔 Polyclonal DyLight 680
anti-CRISPR-Cas9 (C-Term) antibody (DyLight 549)WB, ELISA, FM 宿主: 兔 Polyclonal DyLight 549
anti-CRISPR-Cas9 (C-Term) antibody (DyLight 488)WB, ELISA, FM 宿主: 兔 Polyclonal DyLight 488
anti-CRISPR-Cas9 (C-Term) antibody (DyLight 800)WB, ELISA, FM 宿主: 兔 Polyclonal DyLight 800
anti-CRISPR-Cas9 (C-Term) antibody (Biotin)适用: Streptococcus pyogenes WB, ELISA, FM 宿主: 兔 Polyclonal Biotin
-
-
应用备注
- WB 1:500-2000
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 μg/μL
-
缓冲液
- Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
-
有效期
- 12 months
-
-
- CRISPR-Cas9
-
背景
-
Synonyms: CRISPR, Cas9, CRISPR-associated endonuclease Cas9/Csn1, SpyCas9, SpCas9, csn1
Background: CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.
-
基因ID
- 901176
-
UniProt
- Q99ZW2
抗原
-
