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Ezrin 抗体 (AA 292-464)

Name: Ezrin 抗体 (AA 292-464)
Brand: antibodies-online
SKU: ABIN5542456
Availability: OutOfStock

Cited in 2+ publications. The 小鼠单克隆 anti-Ezrin antibody (Clone 6F1A9) (ABIN5542456) specifically detects Ezrin in WB, ELISA, ICC 和 FACS. The antibody is reactive with 人, 小鼠和猴 samples.

Western blot analysis using EZR mAb against human EZR (AA: 292-464) recombinant protein. (Ezrin 抗体 (AA 292-464))

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng), (Ezrin 抗体 (AA 292-464))

ELISA

Western blot analysis using EZR mAb against HEK293 (1) and EZR (AA: 292-464)-hIgGFc transfected HEK293 (2) cell lysate. (Ezrin 抗体 (AA 292-464))

Western blot analysis using EZR mouse mAb against MCF-7 (1), Hela (2), A431 (3), Hek293 (4), SK-N-SH (5), Jurkat (6), HepG2 (7), NIH/3T3 (8), and Cos7 (9) cell lysate. (Ezrin 抗体 (AA 292-464))

Flow cytometric analysis of MCF-7 cells using EZR mouse mAb (green) and negative control (red). (Ezrin 抗体 (AA 292-464))

FACS

Flow cytometric analysis of Hela cells using EZR mouse mAb (green) and negative control (red). (Ezrin 抗体 (AA 292-464))

FACS

Immunofluorescence analysis of A549 cells using EZR mouse mAb (green). (Ezrin 抗体 (AA 292-464))

7 images

(2 references)

产品编号 ABIN5542456

发货至: 中国

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Quick Overview for Ezrin 抗体 (AA 292-464) (ABIN5542456)

抗原

See all Ezrin (EZR) 抗体

Ezrin (EZR)

适用

人, 小鼠, 猴

宿主

小鼠

克隆类型

单克隆

标记

This Ezrin antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

克隆位点

6F1A9

抗原表位
28

16

10

9

9

9

6

6

5

5

5

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 292-464

原理

EZR Antibody

纯化方法

Purified antibody

免疫原

Purified recombinant fragment of human EZR (AA: 292-464) expressed in E. Coli.

亚型

IgG1
anti-Ezrin (EZR) (AA 1-295) antibody
EZR 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Ezrin (EZR) (pTyr478) antibody
EZR 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Ezrin (EZR) (AA 1-586) antibody
EZR 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-Ezrin (EZR) (pTyr353) antibody
EZR 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Ezrin (EZR) (AA 1-586) antibody
EZR 适用: 人 WB, ELISA, IHC (p), PLA 宿主: 小鼠 Monoclonal 1E11 unconjugated

anti-Ezrin (EZR) antibody
EZR 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal CPTC-Ezrin-1 unconjugated

anti-Ezrin (EZR) (AA 455-586) antibody
EZR 适用: 人, 大鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Ezrin (EZR) (AA 1-251) antibody
EZR 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Ezrin (EZR) antibody
EZR 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Ezrin (EZR) (pThr567) antibody
EZR 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

ELISA: 1/10000
FCM: 1/200 - 1/400
ICC: 1/100 - 1/400

限制

仅限研究用
状态

Liquid

缓冲液

Purified antibody in PBS with 0.05 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Popov, Brazhe, Morozova, Yashin, Bychkov, Nosova, Sutyagina, Brazhe, Parshina, Li, Medyanik, Korzhevskii, Shenkarev, Lyukmanova, Verkhratsky, Semyanov: "Mitochondrial malfunction and atrophy of astrocytes in the aged human cerebral cortex." in: Nature communications, Vol. 14, Issue 1, pp. 8380, (2023) (PubMed).

Popov, Brazhe, Fedotova, Tiaglik, Bychkov, Morozova, Brazhe, Aronov, Lyukmanova, Lazareva, Li, Ponimaskin, Verkhratsky, Semyanov: "A high-fat diet changes astrocytic metabolism to promote synaptic plasticity and behavior." in: Acta physiologica (Oxford, England), pp. e13847, (2022) (PubMed).
抗原

Ezrin (EZR)

别名

EZR

背景

The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.

分子量

69.4 kDa

UniProt

P15311

途径

Maintenance of Protein Location