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ANAPC10 抗体 (AA 1-185)

Name: ANAPC10 抗体 (AA 1-185)
Brand: antibodies-online
SKU: ABIN5542270
Availability: OutOfStock

The 小鼠单克隆 anti-ANAPC10 antibody (Clone 8F1D10) (ABIN5542270) specifically detects ANAPC10 in WB, ELISA, IHC, ICC 和 FACS. The antibody is reactive with 人 samples.

Immunohistochemical analysis of paraffin-embedded colon cancer tissues using ANAPC10 mouse mAb with DAB staining. (ANAPC10 抗体 (AA 1-185))

IHC

Immunofluorescence analysis of Hela cells using ANAPC10 mouse mAb. (ANAPC10 抗体 (AA 1-185))

Immunofluorescence analysis of Hela cells using ANAPC10 mouse mAb (green). (ANAPC10 抗体 (AA 1-185))

Black line: Control Antigen (100 ng),Purple line: Antigen (10 ng), Blue line: Antigen (50 ng), Red line:Antigen (100 ng) (ANAPC10 抗体 (AA 1-185))

ELISA

Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using ANAPC10 mouse mAb with DAB staining. (ANAPC10 抗体 (AA 1-185))

IHC

Western blot analysis using ANAPC10 mAb against human ANAPC10 (AA: 1-185) recombinant protein. (ANAPC10 抗体 (AA 1-185))

Western blot analysis using ANAPC10 mAb against HEK293 (1) and ANAPC10 (AA: 1-185)-hIgGFc transfected HEK293 (2) cell lysate. (ANAPC10 抗体 (AA 1-185))

Western blot analysis using ANAPC10 mouse mAb against Hela (1), MCF-7 (2), SK-Br-3 (3), A431 (4), HEK293 (5), A549 (6), and SPC-A-1 (7) cell lysate. (ANAPC10 抗体 (AA 1-185))

Flow cytometric analysis of Hela cells using ANAPC10 mouse mAb (green) and negative control (red). (ANAPC10 抗体 (AA 1-185))

FACS

9 images

产品编号 ABIN5542270

发货至: 中国

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中国
北京 101111

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Quick Overview for ANAPC10 抗体 (AA 1-185) (ABIN5542270)

抗原

See all ANAPC10 抗体

ANAPC10 (Anaphase Promoting Complex Subunit 10 (ANAPC10))

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This ANAPC10 antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

克隆位点

8F1D10

抗原表位
15

7

4

1

1

1

1
AA 1-185

原理

ANAPC10 Antibody

纯化方法

Purified antibody

免疫原

Purified recombinant fragment of human ANAPC10 (AA: 1-185) expressed in E. Coli.

亚型

IgG1
anti-Anaphase Promoting Complex Subunit 10 (ANAPC10) (AA 1-185) antibody
ANAPC10 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Anaphase Promoting Complex Subunit 10 (ANAPC10) (AA 1-185) antibody
ANAPC10 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Anaphase Promoting Complex Subunit 10 (ANAPC10) (AA 1-185) antibody
ANAPC10 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-Anaphase Promoting Complex Subunit 10 (ANAPC10) (AA 1-185) antibody
ANAPC10 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated

anti-Anaphase Promoting Complex Subunit 10 (ANAPC10) (N-Term) antibody
ANAPC10 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, Bat, 小鸡, Hamster, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated

anti-Anaphase Promoting Complex Subunit 10 (ANAPC10) (N-Term) antibody
ANAPC10 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated

anti-Anaphase Promoting Complex Subunit 10 (ANAPC10) (N-Term) antibody
ANAPC10 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated

anti-Anaphase Promoting Complex Subunit 10 (ANAPC10) (AA 31-130) antibody
ANAPC10 适用: 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated

anti-Anaphase Promoting Complex Subunit 10 (ANAPC10) (AA 31-130) antibody (AbBy Fluor® 750)
ANAPC10 适用: 大鼠 WB, IF (cc), IF (p) 宿主: 兔 Polyclonal AbBy Fluor® 750

anti-Anaphase Promoting Complex Subunit 10 (ANAPC10) (AA 31-130) antibody (AbBy Fluor® 680)
ANAPC10 适用: 大鼠 WB, IF (cc), IF (p) 宿主: 兔 Polyclonal AbBy Fluor® 680

应用备注

ELISA: 1/10000
FCM: 1/200 - 1/400
ICC: 1/200 - 1/1000

限制

仅限研究用
状态

Liquid

缓冲液

Purified antibody in PBS with 0.05 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
抗原

ANAPC10 (Anaphase Promoting Complex Subunit 10 (ANAPC10))

别名

ANAPC10

背景

ANAPC10 is a core subunit of the anaphase-promoting complex (APC), or cyclosome, a ubiquitin protein ligase that is essential for progression through the cell cycle. APC initiates sister chromatid separation by ubiquitinating the anaphase inhibitor securin (PTTG1, MIM 604147) and triggers exit from mitosis by ubiquitinating cyclin B (CCNB1, MIM 123836), the activating subunit of cyclin-dependent kinase-1 (CDK1, MIM 116940) (summary by Wendt et al., 2001 [PubMed 11524682]).

分子量

21.3 kDa

UniProt

Q9UM13

途径

M Phase