CELA1 抗体 (HRP)
Quick Overview for CELA1 抗体 (HRP) (ABIN5541904)
抗原
See all CELA1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects porcine elastase. Cross reactivity against elastase from other tissues and species has not been determined. Immunoelectrophoresis gives a single precipitin arc against anti-Peroxidase, anti-rabbit serum as well as purified and partially purified elastase [porcine pancreas].
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纯化方法
- Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
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免疫原
- Elastase from porcine pancreas
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anti-Chymotrypsin-Like Elastase Family, Member 1 (CELA1) (AA 40-258) antibody
CELA1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Chymotrypsin-Like Elastase Family, Member 1 (CELA1) (AA 159-257) antibodyCELA1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 4H5 unconjugated
anti-Chymotrypsin-Like Elastase Family, Member 1 (CELA1) (AA 44-259) antibodyCELA1 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chymotrypsin-Like Elastase Family, Member 1 (CELA1) antibodyCELA1 适用: 人, 小鼠, 大鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Chymotrypsin-Like Elastase Family, Member 1 (CELA1) (AA 1-258) antibodyCELA1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Chymotrypsin-Like Elastase Family, Member 1 (CELA1) (AA 80-129) antibodyCELA1 适用: 人, 小鼠, Pig, 大鼠, Cow, 兔, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Chymotrypsin-Like Elastase Family, Member 1 (CELA1) (N-Term) antibodyCELA1 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Chymotrypsin-Like Elastase Family, Member 1 (CELA1) antibodyCELA1 适用: 人 WB, EIA, IHC (p) 宿主: 小鼠 Monoclonal 4H5 unconjugated
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应用备注
- Suitable for immunoblotting (Western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. Recommended Dilutions: ELISA : 1/14,000 Western blot : 1/100-1/5,000. This product has been assayed against 1.0 μg of elastase [porcine pancreas] in a standard sandwich ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:200 to 1:1,000 of the reconstitution concentration is suggested.
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限制
- 仅限研究用
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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有效期
- 12 months
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- CELA1 (Chymotrypsin-Like Elastase Family, Member 1 (CELA1))
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别名
- elastase-1
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背景
- Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Elastase is a serine protease which exhibits endopeptidase activity on many proteins, its principal substrate, however, is elastin and it is unique among proteases in its ability to hydrolyse native elastin. It is present in the pancreas, leukocytes and serum. Elastase consists of a single polypeptide chain with a molecular weight of 29.5 kDa. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Like most of the human elastases, elastase 2A is secreted from the pancreas as a zymogen. In other species, elastase 2A has been shown to preferentially cleave proteins after leucine, methionine, and phenylalanine residues. Elastase 1 is not a pancreatic enzyme: clinical literature that describes human elastase 1 activity in the pancreas is actually referring to elastase 2A.
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UniProt
- P00772
抗原
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