Luciferase 抗体
Quick Overview for Luciferase 抗体 (ABIN5541890)
抗原
See all Luciferase 抗体适用
宿主
克隆类型
标记
应用范围
-
-
特异性
- IgG fraction of Polyclonal Rabbit antiserum to Luciferase from Photinus pyralis. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
-
免疫原
- Luciferase isolated and purified from Photinus pyralis. Freund's complete adjuvant is used in the first step of the immunization procedure.
-
亚型
- IgG
-
-
anti-Luciferase antibody
适用: Firefly ELISA, IF (p), IF (cc), IHC (fro), IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Luciferase antibody适用: Firefly WB, IF 宿主: 小鼠 Monoclonal 2C2 unconjugated
anti-Luciferase antibody适用: Photinus pyralis WB, ELISA, IHC, FM 宿主: 山羊 Polyclonal unconjugated
anti-Luciferase antibody适用: Firefly WB, ELISA, IHC 宿主: 小鼠 Monoclonal unconjugated
anti-Luciferase antibody适用: Photinus pyralis WB 宿主: 小鼠 Monoclonal Luci17 unconjugated
anti-Luciferase (C-Term) antibody适用: Photinus pyralis WB 宿主: 山羊 Polyclonal unconjugated
anti-Luciferase (N-Term) antibody适用: Photinus pyralis WB, IHC 宿主: 小鼠 Monoclonal Luci17 unconjugated
anti-Luciferase antibody适用: Photinus pyralis WB, IP 宿主: 小鼠 Monoclonal 2D4 unconjugated
anti-Luciferase antibody适用: Photinus pyralis WB, ELISA 宿主: 山羊 Polyclonal unconjugated
-
-
应用备注
- ELISA. Dot blot. Immunoblotting. Indirect Immunofluorescence. Working Dilutions for Non-precipitating antibody-binding techniques: 1/1000-1/10000. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice.
-
限制
- 仅限研究用
-
-
-
缓冲液
- Buffer System: PBS, pH 7.2 without preservatives and foreign proteins
-
储存液
- Without preservative
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
-
有效期
- 12 months
-
-
- Luciferase
-
背景
- Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
-
UniProt
- P08659
抗原
-
