IgG fraction of Polyclonal Rabbit antiserum to Trypsin from Bovine pancreas. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Species: Bovine.
纯化方法
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
免疫原
Trypsin isolated and purified from Bovine pancreas. Freund's complete adjuvant is used in the first step of the immunization procedure.
ELISA. Dot blot. Immunoblotting. Indirect Immunofluorescence. Can be used in precipitating and non-precipitating antibody-binding assays (such as e.g. , ELISA and Western blotting and Immunofluorescence or histochemical techniques). Working Dilutions in non-precipitating antibody-binding techniques: vary widely, but may be between 1/1000 and 1/7000.
限制
仅限研究用
缓冲液
PBS, pH 7.2 without preservatives and foreign proteins
储存液
Without preservative
储存条件
4 °C,-20 °C
储存方法
Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.