GOT1 抗体 (Biotin)
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Quick Overview for GOT1 抗体 (Biotin) (ABIN5541602)
抗原
See all GOT1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects porcine GOT1. Immunoelectrophoresis give a single precipitin arc against anti-biotin and anti-sheep serum as well as purified and partially purified aspartate transaminase / GOT1 [porcine heart]. Species: Porcine (Pig).
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纯化方法
- Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
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免疫原
- Aspartate aminotransferase / GOT1 from porcine heart
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亚型
- IgG
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anti-Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) (AA 1-413) antibody
GOT1 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C2 unconjugated
anti-Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) (AA 1-413) antibodyGOT1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) (AA 1-413) antibodyGOT1 适用: 人 WB, IP, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) (AA 244-413) antibodyGOT1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) antibodyGOT1 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) (N-Term) antibodyGOT1 适用: 人, 小鼠, 大鼠, Cow, 斑马鱼, 山羊, 豚鼠, 马, 兔, Saccharomyces cerevisiae WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) (AA 1-413) antibodyGOT1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) (AA 2-411) antibodyGOT1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) antibodyKD Validated GOT1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB5585 unconjugated Recombinant Antibody
anti-Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) antibodyGOT1 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
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应用备注
- Western blot : 1/500-1/3,000. ELISA : 1/5,000-1/25,000. This antibody has been assayed against 1.0 μg of aspartate transaminase [porcine heart] in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/20,000 of the reconstitution concentration is suggested.
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限制
- 仅限研究用
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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有效期
- 12 months
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- GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))
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别名
- got1
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背景
- Aspartate aminotransferase (Glutamate oxaloacetate transaminase / GOT1) is a ubiquitous pyridoxal phosphate-dependent enzyme which exists in both mitochondrial and cytosolic forms. The enzyme plays an important role in amino acid metabolism and in the urea and tricarboxylic acid cycles. The 2 isoenzymes are homodimeric. In liver about 80 % of the enzyme activity is mitochondrial in origin, whereas in serum the enzyme activity is largely cytosolic. Although the mitochondrial and soluble forms of GOT are coded by different chromosomes, the 2 show close homology in amino acid sequence and were presumably derived from a common ancestral gene. Serum GOT (with SGPT) levels are usually elevated in states of hepatocellular injury (injury to the liver cells), the highest levels are associated with hepatitis of a viral origin. High levels are also found after myocardial infarction, when SGPT levels are lower.
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UniProt
- P00503
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途径
- Hepatitis C, Monocarboxylic Acid Catabolic Process, Methionine Biosynthetic Process
抗原
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