beta-Galactosidase Tag 抗体 (Biotin)
The Biotin-conjugated 兔 多克隆 anti-beta-Galactosidase Tag antibody (ABIN5541541) specifically detects beta-Galactosidase Tag in WB, EIA, IHC (p) 和 IHC (fro).
产品编号 ABIN5541541
发货至:
中国
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Contact Info
Our Local Distributor
中国
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北京 101111
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Quick Overview for beta-Galactosidase Tag 抗体 (Biotin) (ABIN5541541)
抗原
beta-Galactosidase Tag
适用
请咨询
宿主
兔
克隆类型
多克隆
标记
This beta-Galactosidase Tag antibody is conjugated to Biotin
应用范围
Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
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特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum as well as purified and partially purified Beta-galactosidase (E.coli).
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纯化方法
- Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
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免疫原
- Beta-galactosidase from E.coli
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应用备注
- Western blot : 1/2,000-1/10,000. ELISA : 1/10,000-1/50,000. IHC : 1/1,000-1/5,000. The antibody recognizes both frozen tissue sections, paraffin embedded tissue and 4 % paraformaldehyde fixed tissue for most immunohistochemical analysis : A 1:1,500 dilution has been reported to detect beta-galactosidase in adult rat spinal cord tissue after infection with helper-dependent adenovirus expressing lacZ. In this particular experiment, tissue was perfused with 4 % paraformaldehyde and cryostat-cut (35 μm) to produce free-floating sections. A 1:5,000 dilution has been reported to be successful for staining of brain sections from transgenic mice expressing nuclear beta-galactosidase when assayed by immunofluorescence microscopy. A 1:5,000 dilution has been reported for immunofluorescent staining of methanol fixed, devitellinized Drosophila embryos. A 1:5,000 dilution has been reported to be successful for staining by immunoblot of beta-galactosidase fusion proteins after transfer using a semi-dry transfer apparatus.
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限制
- 仅限研究用
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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有效期
- 12 months
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- beta-Galactosidase Tag
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物质类
- Tag
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UniProt
- P00722
抗原
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