Flow cytometry: 1.2 μg/10e6 cells. ELISA (detection): Clone LU-5E11-A7 as capture antibody. Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.
限制
仅限研究用
状态
Liquid
缓冲液
Phosphate buffered saline, pH 7.2
储存条件
4 °C,-20 °C
储存方法
Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
有效期
12 months
抗原
CRISP3
(Cysteine-Rich Secretory Protein 3 (CRISP3))
别名
crisp3
背景
Cysteine-rich secretory protein 3 (CRISP3) belongs to the cysteine-rich secretory protein family. CRISPs are characterized by a cysteine-rich domain at the COOH terminal that form 8 intramolecular disulfide bonds (1). Mammalian members of the CRISP family are expressed predominantly in the male reproductive tract and are implicated in the process of reproduction from spermiogenesis, posttesticular sperm maturation and capacitation to oocyte-sperm fusion (2). CRISP3 is epithelium-specific and found predominantly in salivary gland, pancreas and prostate, and in less abundance in the epididymis, ovary, thymus and colon. CRISP3 is up-regulated in malignant prostatic epithelium, therefore, it can be used as a potential prostate cancer biomarker (3).