PIN1 抗体 (N-Term)
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Quick Overview for PIN1 抗体 (N-Term) (ABIN5541386)
抗原
See all PIN1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- The antibody detects endogenous levels of total Pin1 protein.
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纯化方法
- Immunoaffinity Chromatography
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免疫原
- Synthetic peptide fom human PIN1, N-terminal epitope
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亚型
- IgG
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应用备注
- Immunohistochemistry on Paraffin Sections: 20 μg/mL. Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 minute at room temperature. Immunocytochemistry/Immunofluorescence: 1/100 - 1/5000. ELISA: 1/5000.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 50 % glycerol Preservatives: 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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有效期
- 12 months
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- PIN1 (Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1))
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别名
- pin1
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背景
- < span class=SHORT_NAME> PPIase Pin1 functions as cell proliferation regulating enzyme.
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UniProt
- Q13526
抗原
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