Endothelial Cells 抗体 (Biotin)
This anti-Endothelial Cells antibody is a 大鼠 单克隆 antibody detecting Endothelial Cells in IHC (fro). Suitable for 人.
Quick Overview for Endothelial Cells 抗体 (Biotin) (ABIN5541247)
抗原
Endothelial Cells
适用
人
宿主
大鼠
克隆类型
单克隆
标记
This Endothelial Cells antibody is conjugated to Biotin
应用范围
Immunohistochemistry (Frozen Sections) (IHC (fro))
克隆位点
1F10
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特异性
- This antibody recognizes an antigen on human continuous blood endothelial cells which are fully differentiated. The antibody staining is restricted to endothelial cells and does not sta in any other cell in tissues or in peripheral blood. On sinusoidal endothelial cells of the liver or lymphatic organs the antigen is inconsistently expressed or absent.
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免疫原
- Cultured HUVECs
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亚型
- IgG2a
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anti-Endothelial Cells antibody
适用: Pig IHC (fro), EIA 宿主: 小鼠 Monoclonal PE1 unconjugated
anti-Endothelial Cells antibody适用: 人 IHC (fro) 宿主: 大鼠 Monoclonal 1F10 unconjugated
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应用备注
- Immunohistochemistry on Frozen sections: 2 μg/mL (1:200). Positive control: Human placenta. Does not react on routinely processed paraffin sections.
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限制
- 仅限研究用
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缓冲液
- 0.01 % thimerosal Stabilizers: 10 mg/mL bovine serum albumin
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储存条件
- 4 °C,-20 °C
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储存方法
- Store lyophilized at 2-8°C. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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有效期
- 12 months
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- Endothelial Cells
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背景
- The antigen is found in variable numbers on freshly isolated umbilical vein endotheli al cells (HUVECs). Antigen expression on cultured HUVECs is dependent on the culture conditions. Best results were obtained (40 - 70 % pos.) by daily renewal of FCS containing medium supplemented with endothelial cell conditioned supernatant whereas commercia lly available endothelial cell media apparently have no influence on the antigen expression.
抗原
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