CEACAM5/6 抗体
This anti-CEACAM5/6 antibody is a 小鼠 单克隆 antibody detecting CEACAM5/6 in FACS, IHC (fro), WB, EIA, ICC 和 IF. Suitable for 人. This Primary Antibody has been cited in 2+ publications.
(2 references)Quick Overview for CEACAM5/6 抗体 (ABIN5541175)
抗原
CEACAM5/6
(Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5/6 (CEACAM5/6))
适用
人
宿主
小鼠
克隆类型
单克隆
标记
This CEACAM5/6 antibody is un-conjugated
应用范围
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Enzyme Immunoassay (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF)
克隆位点
MUS
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特异性
- This antibody reacts to CD66c/e. Species: Human.
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纯化方法
- Affinity Chromatography on Protein G
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免疫原
- MUS was generated by immunisation of BALB/c mice with CEA partially purified from a perchloric acid extract from liver metastases of colonic tumors (3).
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亚型
- IgG1
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anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5/6 (CEACAM5/6) antibody
CEACAM5/6 适用: 人 FACS, IHC (fro), WB, cELISA, ELISA 宿主: 小鼠 Monoclonal MUS unconjugated
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应用备注
- Flow Cytometry: 1.2 μg/10 6 cells. ELISA: 1/200-1/400. Cell based ELISA with intakt, transiently transfected cells: 1/200. Western blot: 4 μg/mL. Immunohistochemistry: 1-2 μg/10 cells (on Cryosections).
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Phosphate buffered saline, pH 7.2
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储存条件
- 4 °C,-20 °C
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储存方法
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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有效期
- 12 months
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: "Extracellular IgC2 constant domains of CEACAMs mediate PI3K sensitivity during uptake of pathogens." in: PLoS ONE, Vol. 7, Issue 6, pp. e39908, (2012) (PubMed).
: "RNA interference suppression of MUC1 reduces the growth rate and metastatic phenotype of human pancreatic cancer cells." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 12, Issue 10, pp. 2976-87, (2006) (PubMed).
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: "Extracellular IgC2 constant domains of CEACAMs mediate PI3K sensitivity during uptake of pathogens." in: PLoS ONE, Vol. 7, Issue 6, pp. e39908, (2012) (PubMed).
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- CEACAM5/6 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5/6 (CEACAM5/6))
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别名
- cd66c/e
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背景
- CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family (1). The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/CEACAM5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker (2). CEACAM6 expression is strongly upregulated already during early stages of adenocarcinoma formation (3). The function of CEA family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria (1).
抗原
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