COL6 抗体
Quick Overview for COL6 抗体 (ABIN5541083)
抗原
See all COL6 抗体适用
宿主
克隆类型
标记
应用范围
-
-
特异性
- Human Collagen type VI: 100 % binding in RIA at 1/10,000 dilution.
-
纯化方法
- Affinity Chromatography.
-
免疫原
- Purified collagen type VI from human placenta.
-
-
anti-Collagen, Type VI (COL6) antibody
COL6 适用: 人, Cow WB, ELISA, IHC, IP, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type VI (COL6) antibodyCOL6 适用: 人 ELISA, IHC, IHC (p), IHC (fro), ICC, DB 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type VI (COL6) antibodyCOL6 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type VI (COL6) antibody (Biotin)COL6 适用: 人 ELISA 宿主: 山羊 Polyclonal Biotin
anti-Collagen, Type VI (COL6) (AA 1-336) antibodyCOL6 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type VI (COL6) antibodyCOL6 适用: 人, Cow WB, ELISA, IHC, IP, DB 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type VI (COL6) antibodyCOL6 适用: 人, 哺乳动物 WB, ELISA, IP, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- ELISA: 1/200 (OD ≥ 500). RIA. Immunofluorescence: 1/80. Immunohistology on Frozen and Paraffin Embedded Sections. For paraffin: 1/400-1/1000, 60 min at RT or 2-8 °C over night for human collagen type VI, lower dilution is recommended for other species). Positive Control: Human placenta. Pretreatment : After de-waxing the tissue slices are treated with 0.2 % hyaluronidase (app. 300 U/mg) in TBS 15 min at 37 °C. There after non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
缓冲液
- PBS containing no BSA or preservative.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
-
有效期
- 12 months
-
-
- COL6 (Collagen, Type VI (COL6))
-
别名
- collagen type vi
-
背景
- Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Collagen type VI is found as 5-10 nm diameter microfibrils (periodicity 100 nm) e.g. in placenta, skin, cartilage and cornea.
-
UniProt
- P12109
抗原
-
