This antibody detects rabbit aldolase. Cross reactivity against aldolase from human tissues is known. Crossactivity against aldolase from other tissues and species may also occur. Immunoelectrophoresis gives a single precipitin arc against anti-goat serum, purified and partially purified aldolase [Rabbit muscle]. It has been reported that this antibody can detect human Aldolase on immunoblot showing a 41 kDa band in lysates from MCF7, NMB231 and HBL100 cell lines. Species: Rabbit, Human.
纯化方法
Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Western blot :1/500-1/5,000. Immunoprecipitation : 1/100. Use approximately 5 μL of antibody to immunoprecipitate 50 μL of protein lysate. ELISA : 1/5000-1/20,000. This antibody has been assayed against 1.0 μg of aldolase [rabbit muscle] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 to 1/2,000 of the reconstitution concentration is suggested.
限制
仅限研究用
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C,-20 °C
储存方法
Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Avoid repeated freezing and thawing. Shelf life: one year from despatch.