ALDOA 抗体 (Biotin)
Quick Overview for ALDOA 抗体 (Biotin) (ABIN5541022)
抗原
See all ALDOA 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects rabbit aldolase. Cross reactivity against aldolase from other tissues and species may also occur. Immunoelectrophoresis gives a single precipitin arc against anti-goat serum, purified and partially purified aldolase [rabbit muscle]. It has been reported that this antibody can detect human Aldolase on immunoblot showing a 41 kDa band in lysates from MCF7, NMB231 and HBL100 cell lines. Species: Rabbit, Human.
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纯化方法
- Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
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免疫原
- Aldolase from rabbit muscle
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应用备注
- Western blot :1/500-1/5,000. Immunoprecipitation : 1/100. Use approximately 5 μL of antibody to immunoprecipitate 50 μL of protein lysate. ELISA : 1/5,000-1/20,000. This antibody has been assayed against 1.0 μg of aldolase [rabbit muscle] in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/16,000 of the reconstitution concentration is suggested for this product.
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限制
- 仅限研究用
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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有效期
- 12 months
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- ALDOA (Aldolase A, Fructose-Bisphosphate (ALDOA))
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别名
- aldolase a,aldoa
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UniProt
- P00883
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途径
- Ribonucleoside Biosynthetic Process
抗原
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