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Doublecortin 抗体

This anti-Doublecortin antibody is a 小鸡 多克隆 antibody detecting Doublecortin in IF, ICC 和 IHC (fro). Suitable for 人 和 小鼠.
产品编号 ABIN5540930
发货至: 中国

Quick Overview for Doublecortin 抗体 (ABIN5540930)

抗原

See all Doublecortin (DCX) 抗体
Doublecortin (DCX)

适用

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人, 小鼠

宿主

  • 95
  • 14
  • 4
小鸡

克隆类型

  • 91
  • 22
多克隆

标记

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This Doublecortin antibody is un-conjugated

应用范围

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Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • 纯化方法

    Affinity Chromatography

    免疫原

    Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. Production: These synthetic peptides corresponded to different regions of the Doublecortin gene product, but are shared between the Human (CAA06617.1, NCBI) and Mouse (AAT58219.1, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.2 mg/ml. Finally, equal volumes of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

    亚型

    Ig Fraction
  • 应用备注

    Immunofluorescence/ Immnunocytochemistry. Immunohistochemistry. Quality Control: This anti-peptide antibody mixture was validated by Immunohistochemistry (at a concentration of 2 μg/mL) using Fluorescein Conjugated Affinity-purified Goat anti-Chicken IgY antibody (Cat.-No AP31795FCN, 1/5000 dilution) as a secondary antibody. Recommended Dilutions : 1/1000-1/2000 for Immunohistochemistry and Immunocytochemistry using 2 % paraformaldehyde-fixed tissues or cells.

    限制

    仅限研究用
  • 状态

    Liquid

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Store undiluted at 2-8°C. This product is photosensitive and should be protected from light. Shelf life: one year from despatch.

    有效期

    12 months
  • 抗原

    Doublecortin (DCX)

    别名

    doublecortin

    UniProt

    O43602
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