This antibody detects porcine esterase. Cross reactivity against esterase from other tissues and species may occur but have not been specifically determined. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified Esterase [porcine liver].
纯化方法
Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Suitable for immunoblotting (Western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays. Recommended Dilutions: Western blot : 1/1000-1/5000 Immunohistochemistry. ELISA : 1/14000. This product has been assayed against 1.0 μg of esterase [porcine liver] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,500 of the reconstitution concentration is suggested.
限制
仅限研究用
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C,-20 °C
储存方法
Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.