Actin 抗体
Quick Overview for Actin 抗体 (ABIN5540756)
抗原
See all Actin 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Affinity Chromatography
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免疫原
- Chickens were immunized with actin purified from Rabbit muscle. After epeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column. The final product contains 50% glycerol (v/v), purified anti-Actin IgY fraction (10 mg/ml) spiked with 25 mug/ml of affinity purified anti-Actin IgY, and then filter-sterilized.
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亚型
- Ig Fraction
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anti-Actin antibody
适用: 植物 WB 宿主: 小鼠 Monoclonal Q30 unconjugated
anti-Actin (AA 165-260) antibody适用: 人, 大鼠, 小鼠, 兔, 犬 WB, ELISA, IHC (p), IF (cc), IF (p), FACS, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Actin antibody适用: 人, 大鼠, 小鼠, 小鸡, 犬, Cow, Pig, 豚鼠 WB, ELISA, IHC (p), IF (cc), IF (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Actin (C-Term) antibody适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Actin antibody适用: Oryza sativa, Barley, Arabidopsis, Nicotiana tabacum WB 宿主: 兔 Polyclonal unconjugated
anti-Actin (AA 367-377) antibody适用: 人, 大鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Actin (AA 21-70) antibody适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Actin (AA 38-67), (N-Term) antibody适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Actin (AA 189-218) antibody适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Quality Control: This anti-Actin preparation was validated by Western blotting on nitrocellulose membranes (at a concentration of 1 μg/mL) using HRP-Conjugated Goat anti-Chicken IgY (1/5000 dilution, Cat.-No AP31795HR-N) as the secondary reagent. Recommended Dilutions: 1/1000-1/2000 for Western blot analysis of Nitrocellulose membranes. Please note that these dilutions are meant to serve as starting points, and that optimal dilutions may vary. Do Not use this product with PVDF, or other nylon-based membranes, because this often produces unacceptably high backgrounds in chemiluminescence studies.
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限制
- 仅限研究用
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状态
- Liquid
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store undiluted at -20°C This product is photosensitive and should be protected from light. Shelf life: one year from despatch.
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有效期
- 12 months
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- Actin
抗原
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