TXN 抗体
Quick Overview for TXN 抗体 (ABIN5540698)
抗原
See all TXN 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody reacts with thioredoxin.
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交叉反应 （详细）
- Does not treact with Mouse (NIH/3T3, WR19L, Ba/F3) and Rat (PC12).
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纯化方法
- Protein A agarose
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免疫原
- Recombinant full-length human thioredoxin
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亚型
- IgG2a
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anti-Thioredoxin (TXN) (AA 1-105) antibody
TXN 适用: 人 WB, ELISA, IP, IF 宿主: 小鼠 Monoclonal 2A7 unconjugated
anti-Thioredoxin (TXN) (AA 2-105) antibodyTXN 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Thioredoxin (TXN) (AA 1-105) antibodyTXN 适用: 人 WB, IHC, IP, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Thioredoxin (TXN) (AA 1-105) antibodyTXN 适用: 人 WB 宿主: 小鼠 Monoclonal 3# unconjugated
anti-Thioredoxin (TXN) (AA 66-94), (C-Term) antibodyTXN 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB42816 unconjugated
anti-Thioredoxin (TXN) (AA 51-105) antibodyTXN 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Thioredoxin (TXN) (AA 1-105) antibodyTXN 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Thioredoxin (TXN) (AA 1-105) antibodyTXN 适用: 人 FACS 宿主: 兔 Polyclonal unconjugated
anti-Thioredoxin (TXN) (AA 1-105) antibodyTXN 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Thioredoxin (TXN) antibodyTXN 适用: 人 WB, ELISA 宿主: 兔 Monoclonal 3G7 unconjugated Recombinant Antibody
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应用备注
- Western blot: 1 mg/mL for chemiluminescence detection system. For details see protocol below.
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实验流程
- SDS-PAGE & Western Blotting 1) Wash the cells 3 times with PBS and suspend with 10 volume of cold Lysis buffer (50 mM Tris-HCl, pH 7.2, 250 mM NaCl, 0.1 % NP-40, 2 mM EDTA, 10 % glycerol) containing appropriate protease inhibitors. Incubate it at 4 o C with rotating for 30 minutes, then sonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4 o C and transfer the supernatant to another tube. Measure the protein concentration of the supernatant and add the cold Lysis buffer to make 8 mg/mL solution. 3) Mix the sample with equal volume of Laemmli's sample buffer. 4) Boil the samples for 2 minutes and centrifuge. Load 10 μ L of the sample per lane in a 1 mm thick SDS-polyacrylamide gel for electrophoresis. 5) Blot the protein to a polyvinylidene difluoride (PVDF) membrane at 1 mA/cm 2 for 1 hour in a semi-dry transfer system (Transfer Buffer: 25 mM Tris, 190 mM glycine, 20 % MeOH). See the manufacture's manual for precise transfer procedure. 6) To reduce nonspecific binding, soak the membrane in 10 % skimmed milk (in PBS, pH 7.2) for 1 hour at room temperature, or overnight at 4 o C. 7) Incubate the membrane with primary antibody diluted with PBS, pH 7.2 containing 1 % skimmed milk as suggest in the APPLICATIONS for 1 hour at room temperature. (The concentration of antibody will depend on condition.) 8) Wash the membrane with PBS-T [0.05 % Tween-20 in PBS] (5 minutes x 6 times). 9) Incubate the membrane with the 1:10,000 HRP-conjugated anti-mouse IgG diluted with 1 % skimmed milk (in PBS, pH 7.2) for 1 hour at room temperature. 10) Wash the membrane with PBS-T (5 minutes x 6 times). 11) Wipe excess buffer on the membrane, then incubate it with appropriate chemilumin escence reagent for 1 minute. Remove extra reagent from the membrane by dabbing with paper towel, and seal it in plastic wrap. 12) Expose to an X-ray film in a dark room for 5 minutes. Develop the film as usual. The condition for exposure and development may vary. (Positive controls for Western blotting Raji, HeLa)
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- PBS containing 50 % glycerol, pH 7.2. No preservative is contained.
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储存液
- Azide free
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储存条件
- -20 °C
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储存方法
- Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
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- TXN (Thioredoxin (TXN))
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别名
- thioredoxin,trx1
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背景
- Thioredoxin (TRX) is a small ubiquitous protein (12 kDa) which is exist in a wide activity of prokaryotic and eukaryotic cells. TRX is activated by reducing its disulfide group (-S-S- → 2SH) between cysteine residues within the conserved active site sequence: -Cys-Gly-Pro-Cys-. The reduction of TRX is specifically catalyzed by "TRX reductase", a FAD and selenocystein containing protein, using NADPH. Once reduced, TRX in turn reduces disulfide bonds of various proteins to regulate the activity of the protein (redox regulation). TRX has multiple biological function Activation of transcripti on factors such as NF- κ B and glucocotricoid receptor, anti-apoptotic function, stimulation of cytokine expression, tissue protection from ischemia-reperfusion injury, cytoprotection from cytotoxic and DNA-damaging agents, proliferation, and drug resistance in certain malignancies. A part of these functions are due to TRX which exported out of the cell where it has additional ability to stimulate cell growth, cytoprotection, and co-cytokine activity. It is said that some of above TRX functions are related to response against the cellular "stresses". In fact, TRX is translocated from the cytosol into the nucleus and/or highly expressed by various stress such as UV irradiation and viral infection. So, it is suggested that TRX may have a role in the cellular responses against the stresse s through the regulation of intracellular redox (oxidation-reduction) atatus.
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UniProt
- P10599
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途径
- Carbohydrate Homeostasis, Cell RedoxHomeostasis
抗原
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