TXN 抗体
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北京 101111
Quick Overview for TXN 抗体 (ABIN5540697)
抗原
See all TXN 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody reacts with E. coli Trx and Trx-tag-fusion protein. I detects 12KD of E.coli TrxA by Western blotting on total cell lysate from induced E. coli harboring Trx-expression vector.
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纯化方法
- Protein-A Sepharose
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免疫原
- E. coli thioredoxin (trxA) (109 aa)
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亚型
- IgG1
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anti-Thioredoxin (TXN) (AA 1-105) antibody
TXN 适用: 人 WB, ELISA, IP, IF 宿主: 小鼠 Monoclonal 2A7 unconjugated
anti-Thioredoxin (TXN) (C-Term) antibodyTXN 适用: 人 WB, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Thioredoxin (TXN) (AA 2-105) antibodyTXN 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Thioredoxin (TXN) (AA 1-105) antibodyTXN 适用: 人 WB, IHC, ICC, IP, FACS 宿主: 兔 Polyclonal unconjugated
anti-Thioredoxin (TXN) (AA 1-105) antibodyTXN 适用: 人 WB 宿主: 小鼠 Monoclonal 3# unconjugated
anti-Thioredoxin (TXN) (AA 66-94), (C-Term) antibodyTXN 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB42816 unconjugated
anti-Thioredoxin (TXN) (AA 51-105) antibodyTXN 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Thioredoxin (TXN) antibodyTXN 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Thioredoxin (TXN) antibodyTXN 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 25GB7270 unconjugated
anti-Thioredoxin (TXN) (AA 1-105) antibodyTXN 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
- Western blot: 1:1000 for chemiluminescence detection system. Can be used for West-Western blot.
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实验流程
- 1) Boil all samples for 3~5 minutes. Load 10 μL of cell lysate or tissue homogenate (5~20 μg total protein) to each well of an SDS - polyacrylamide gel and electrophorese in a 1 mm thick gel. 2) Transfer to a p olyvinylidene difluoride (PVDF) membrane at 10V f or 1 hour in a semi-dry transfer system. (Transfer Bu ffer: 25 mM Tris, 190 mM glycine, 20 % MeOH). 3) The transferred protein s can be visualized by staining the membrane for 1 minute with Ponceau S. Rinse the membrane with PBS. 4) Non-specific binding sites are blocked by immersing the membrane in Block Ace TM for 1 hour at room t emperature, or for overnight at 4 ° C. 5) Incubate in primary an t ibody diluted as suggested in the APPLICATIONS for 1 hour at room temperature. (The optimal antibody concentration will depend on the experimental variables and the abundance of the antigen.) 6) Wash the membrane 3 times with PBS, 0.2 % Tween20 for 5~10 minutes per wash. 7) Incubate in Horseradish Peroxidase conjugated goat anti-mouse diluted 1:10 000 in PBS, 0.2 % Tween20 for 45 minutes at room temperature. 8) Wash the membrane 3 times with PBS, 0. 2 % Tween20 for 10 minutes per wash. 9) Incubate in Ame rsham ECL Reagent for 1 minute. Drain membrane, remove excess ECL Reagent by dabbing with a Kimwipe, and seal in plastic wrap. 10) Expose to ECL hyperf ilm in a dark room for 30 seconds. Develop as usual for autoradiogram or X-ray. The conditions for development and exposure may vary.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- PBS containing 50 % glycerol. Contains no preservatives
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储存液
- Without preservative
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储存条件
- -20 °C
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储存方法
- Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
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- TXN (Thioredoxin (TXN))
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别名
- thioredoxin,trx1
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背景
- The thioredoxin (Trx) fusion E. coli expression system is avail a ble to offer soluble expression of normally insoluble o r difficult to express proteins. LaVallie et al. 1) reported that a number of mammalian cytokines and growth factors, when expressed as C-terminal trxA fusion proteins, stayed remarkably soluble in the E. coli cytoplasm under certain conditions.
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UniProt
- P10599
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途径
- Carbohydrate Homeostasis, Cell RedoxHomeostasis
抗原
-
