s100a4 抗体
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北京 101111
Quick Overview for s100a4 抗体 (ABIN5540655)
抗原
See all s100a4 (S100A4) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes Human S100 Calcium Binding Protein A4 (S100A4). Other species not tested.
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纯化方法
- Affinity Chromatography on Protein G
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免疫原
- Genetic immunisation with cDNA encoding S100A4. Selection: based on recognition of the complete native protein expressed on transfected mammalian cells.
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亚型
- IgG1
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应用备注
- Flow cytometry: 1.2 μg/10 6 cells. ELISA: 1/200-1/400. ELISA (capture): Clone NJ-4F3-D1 as detection antibody. ELISA (detection): Clone NJ-5C6-A3 as capture antibody. Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Phosphate buffered saline, pH 7.2
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储存条件
- 4 °C,-20 °C
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储存方法
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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有效期
- 12 months
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- s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))
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别名
- s100a4
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背景
- S100 calcium binding protein A4 (S100A4) is a member of the S100 family of calcium-binding proteins that contain two Ca(2+)-binding sites including a canonical EF-hand motif. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells. S100A4 interacts with cytoskeletal proteins and enhances metastasis of several types of cancer cells. It is secreted by unknown mechanisms, thus, paracrinely stimulating a variety of cellular responses, including angiogenesis and neuronal growth (1). S100A4 has been shown to be a prognostic marker in a number of human cancers, including esophageal-squamous cancers, non-small lung cancers, primary gastric cancers, malignant melanomas, prostate cancers, bladder cancers, and pancreatic carcinomas. The universality of S100A4 expression in a variety of cancers illustrates the potential use of S100A4 as a marker for tumor metastasis and disease progression (2).
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UniProt
- P26447
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途径
- S100 Proteins
抗原
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