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s100a4 抗体

This anti-s100a4 antibody is a 小鼠 单克隆 antibody detecting s100a4 in FACS, EIA, ELISA (Capture) 和 ELISA (Detection). Suitable for 人.
产品编号 ABIN5540655
发货至: 中国

Quick Overview for s100a4 抗体 (ABIN5540655)

抗原

See all s100a4 (S100A4) 抗体
s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This s100a4 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Enzyme Immunoassay (EIA), ELISA (Capture), ELISA (Detection)

克隆位点

NJ-4F3
  • 特异性

    Recognizes Human S100 Calcium Binding Protein A4 (S100A4). Other species not tested.

    纯化方法

    Affinity Chromatography on Protein G

    免疫原

    Genetic immunisation with cDNA encoding S100A4. Selection: based on recognition of the complete native protein expressed on transfected mammalian cells.

    亚型

    IgG1
  • 应用备注

    Flow cytometry: 1.2 μg/10 6 cells. ELISA: 1/200-1/400. ELISA (capture): Clone NJ-4F3-D1 as detection antibody. ELISA (detection): Clone NJ-5C6-A3 as capture antibody. Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Phosphate buffered saline, pH 7.2

    储存条件

    4 °C,-20 °C

    储存方法

    Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

    有效期

    12 months
  • 抗原

    s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

    别名

    s100a4

    背景

    S100 calcium binding protein A4 (S100A4) is a member of the S100 family of calcium-binding proteins that contain two Ca(2+)-binding sites including a canonical EF-hand motif. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells. S100A4 interacts with cytoskeletal proteins and enhances metastasis of several types of cancer cells. It is secreted by unknown mechanisms, thus, paracrinely stimulating a variety of cellular responses, including angiogenesis and neuronal growth (1). S100A4 has been shown to be a prognostic marker in a number of human cancers, including esophageal-squamous cancers, non-small lung cancers, primary gastric cancers, malignant melanomas, prostate cancers, bladder cancers, and pancreatic carcinomas. The universality of S100A4 expression in a variety of cancers illustrates the potential use of S100A4 as a marker for tumor metastasis and disease progression (2).

    UniProt

    P26447

    途径

    S100 Proteins
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