Metabotropic Glutamate Receptor 1 抗体
Quick Overview for Metabotropic Glutamate Receptor 1 抗体 (ABIN5540470)
抗原
See all Metabotropic Glutamate Receptor 1 (GRM1) 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Affinity Chromatography
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免疫原
- Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. Production: These synthetic peptides corresponded to different regions of the metabotropic glutamate receptor type 1 gene product, but are shared between the human (NP_000829.2, NCBI) and mouse (NP_058672.1, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
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亚型
- Ig Fraction
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应用备注
- Quality Control: This anti-peptide antibody mixture was validated by Immunohistochemistry (at 1/2000 dilution) using Fluorescein Conjugated Affinity-purified Goat anti-Chicken IgY antibody (Cat.-No AP31795FCN, 1/5000 dilution) as a secondary antibody. Recommended Dilutions: 1/1000-1/2000 for Immunohistochemistry and Immunocytochemistry using 2 % paraformaldehyde-fixed tissues or cells.
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限制
- 仅限研究用
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状态
- Liquid
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 2-8°C. This product is photosensitive and should be protected from light. Shelf life: one year from despatch.
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有效期
- 12 months
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- Metabotropic Glutamate Receptor 1 (GRM1) (Glutamate Receptor, Metabotropic 1 (GRM1))
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别名
- mglur1a,grm1a
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UniProt
- Q13255
抗原
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