PRAME 抗体 (C-Term)
Quick Overview for PRAME 抗体 (C-Term) (ABIN5540467)
抗原
See all PRAME 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 321-509, C-Term
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特异性
- This antibody detects PRAME / MAPE at C-term.
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纯化方法
- Protein A chromatography
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免疫原
- Highly pure (> 95%) recombinant human PAME /MAPE C-terminal end (Met321-Asn509) derived from E. coli
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亚型
- IgG
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anti-Preferentially Expressed Antigen in Melanoma (PRAME) (AA 416-502) antibody
PRAME 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Preferentially Expressed Antigen in Melanoma (PRAME) (AA 151-250) antibodyPRAME 适用: 人, 小鼠 ELISA, FACS, IF (cc), IF (p), IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Preferentially Expressed Antigen in Melanoma (PRAME) (AA 1-509) antibodyPRAME 适用: 人 WB, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Preferentially Expressed Antigen in Melanoma (PRAME) (AA 1-509) antibodyPRAME 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Preferentially Expressed Antigen in Melanoma (PRAME) (AA 1-509) antibodyPRAME 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Preferentially Expressed Antigen in Melanoma (PRAME) (AA 416-502) antibodyPRAME 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Preferentially Expressed Antigen in Melanoma (PRAME) (AA 476-502), (C-Term) antibodyPRAME 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB18763 unconjugated
anti-Preferentially Expressed Antigen in Melanoma (PRAME) (AA 71-120) antibodyPRAME 适用: 人, 马, Pig, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-Preferentially Expressed Antigen in Melanoma (PRAME) (AA 460-510) antibodyPRAME 适用: 人, Cow, 马, 猴, 犬 WB 宿主: 兔 Polyclonal unconjugated
anti-Preferentially Expressed Antigen in Melanoma (PRAME) (N-Term) antibodyPRAME 适用: 人, 大鼠, Cow, Pig, 猴 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Western blot: 1-5 μg/mL. ELISA: 0.1-1 μg/mL.
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限制
- 仅限研究用
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缓冲液
- PBS
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储存条件
- 4 °C,-20 °C
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储存方法
- Store lyophilized at 2-8°C for 6 month or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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有效期
- 12 months
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- PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))
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别名
- prame,mape
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背景
- PRAME/MAPE/OIP4 is a germinal tissue-specific gene that is also expressed at high levels in haematological malignancies and solid tumors. The physiological functions of PRAME in normal and tumor cells are unknown, although a role in the regulation of retinoic acid signaling has been proposed. Sequence homology and structural predictions suggest that PRAME is related to the Leucine-rich repeat (LRR) family of proteins, which have diverse functions. PRAME, or "preferentially expressed antigen in melanoma", was originally identified as a gene encoding a HLA-A24 restricted antigenic peptide presented to autologous tumor-specific cytotoxic T lymphocytes derived from a patient with melanoma. PRAME is synonymous with MAPE (melanoma antigen preferentially expressed in tumors) and OIP4 (OPA-interacting protein 4), and its expression profile defines it as a cancer-testis antigen. Cancer-testis antigens (CTAs) are encoded by non-mutated genes expressed at high levels in germinal tissues and tumors, but which are absent from or detected at low levels in other tissues. PRAME may be somewhat different to other cancer-testis antigens in that it shows some expression in normal tissues such as ovary, adrenal, placenta and endometrium. The C-terminus of human PRAME (amino acids 453-509) was also identified to bind Neisseria gonorrhoeae opacity factors, in this case the OPA-P protein. Thus PRAME is also known as OIP4 (OPA interacting protein).
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途径
- Retinoic Acid Receptor Signaling Pathway, Nuclear Hormone Receptor Binding
抗原
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