LYZ 抗体
Quick Overview for LYZ 抗体 (ABIN5540464)
抗原
See all LYZ 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- The reactivity of the antiserum is restricted to lysozyme. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal human milk and tears a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified lysozyme. No reaction is obtained with any other plasma protein component or serum. Cross-reactivity: The antiserum does not cross-react with any other component of human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
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纯化方法
- Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt precipitation and purification of the IgG fraction by DEAE-chromatography
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免疫原
- Highly purified lysozyme is isolated from pooled milk.Freund's complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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anti-Lysozyme (LYZ) (AA 1-100) antibody
LYZ 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lysozyme (LYZ) (AA 19-148) antibodyLYZ 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lysozyme (LYZ) antibodyLYZ 适用: 人 ICC, FACS, MS 宿主: 小鼠 Monoclonal LZ598-10G9 unconjugated
anti-Lysozyme (LYZ) antibodyLYZ 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Lysozyme (LYZ) (AA 24-144) antibodyLYZ 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lysozyme (LYZ) (AA 22-143) antibodyLYZ 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lysozyme (LYZ) (AA 19-148) antibodyLYZ 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Lysozyme (LYZ) (AA 18-147) antibodyLYZ 适用: Cow WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Lysozyme (LYZ) antibodyLYZ 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1C9 unconjugated
anti-Lysozyme (LYZ) antibodyLYZ 适用: 人 WB, IF 宿主: 小鼠 Monoclonal 1D8 unconjugated
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应用备注
- The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates in radioimmunoassay for the production of immunoconjugates with a selected marker to prepare immunoaffinity adsorbents by coupling to an artificial carrier in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent in Western blotting. This product is not pre-diluted. Recommended Working Dilutions : Histochemical and cytochemical: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000. Immunohistochemistry on Paraffin Sections: 1/400.
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限制
- 仅限研究用
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缓冲液
- PBS, pH 7.2 Preservatives: None Stabilizers:
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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有效期
- 12 months
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- LYZ (Lysozyme (LYZ))
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别名
- lysozyme c
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背景
- Lysozyme, a bacteriolytic enzyme, has a molecular weight of about 15,000. Its concentration in plasma is low in milk the concentration is reported to be between 5 and 220 mg/mL. Also tears contain a considerable amount of lysozyme.
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UniProt
- P00698
抗原
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