This antibody detects Human GFRa-3 and doesn't cross-react with hGFRa-1, hGFRa-2, hGFRa-4. Other species not tested.
纯化方法
Affinity Chromatography
免疫原
Recombinant His-tagged human GFRa-3 protein produced using CHO-based. For production of hGFRa-3, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
ELISA: 1/160000-1/320000. Western blot: 1/5000-1/10000. Immunofluorescence: 1/500-1/3000.
限制
仅限研究用
状态
Liquid
缓冲液
Concentrated Ammonium Sulphate in PBS pH 7.4
储存条件
4 °C
储存方法
Upon receipt, store at 2-8°C. As product is (NH4) 2 SO 4 precipitate, mix well by pipetting or vortexing prior use. Shelf life: One year from despatch.
抗原
GFRA3
(GDNF Family Receptor alpha 3 (GFRA3))
别名
gfra3,gdnfr-alpha 3
背景
Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The GFRa-1 is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor (www.ncbi.nlm.nih.gov/gene/2674).