This antibody detects PEP1 [maize leaves]. Cross reactivity against Phospho-enol-pyruvate carboxylase from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified Phospho-enol-pyruvate carboxylase [maize].
纯化方法
Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
免疫原
Phospho-enol-pyruvate carboxylase / PEP1 from maize
Western Blot : 1/1,000-1/5,000. ELISA : 1/14,000. This product has been assayed against 1.0 μg of Phospho-enol-pyruvate carboxylase [maize] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 to 1/6,000 of the reconstitution concentration is suggested. Immunohistochemistry : Paraffin (FFPE) or Frozen sections 1/100 suggested to begin.
限制
仅限研究用
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C,-20 °C
储存方法
Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.