PRNP 抗体
Quick Overview for PRNP 抗体 (ABIN5540135)
抗原
See all PRNP 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Purified
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免疫原
- Chickens were immunized with a recombinant fragment of the human prion protein emulsified with Freund's complete and incomplete adjuvants. After multiple injections, eggs were collected from the hens, and IgY fractions were prepared from the yolks. Affinity-purified antibodies were then prepared using an agarose column to which the recombinant protein fragments were covalently attached.Finally, affinity purified antibodies (50 ug/ml final concentration) were mixed with the IgY fraction (10 mg/ml final concentration) in a mixture containing glycerol (50% v/v) (to prevent freezing at -20°C) and 0.02% Sodium Azide.
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亚型
- Ig Fraction
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anti-Prion Protein (PRNP) (AA 23-230) antibody
PRNP 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Prion Protein (PRNP) (AA 23-230) antibodyPRNP 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 3G10G9 unconjugated
anti-Prion Protein (PRNP) antibodyPRNP 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Prion Protein (PRNP) antibodyPRNP 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Prion Protein (PRNP) (AA 23-230) antibodyPRNP 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Prion Protein (PRNP) (AA 23-64) antibodyPRNP 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Prion Protein (PRNP) antibodyPRNP 适用: 人, 大鼠, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Prion Protein (PRNP) antibodyPRNP 适用: 人, Hamster WB, ELISA, IHC, IP 宿主: 小鼠 Monoclonal 3F4 unconjugated Recombinant Antibody
anti-Prion Protein (PRNP) (Prion) antibodyPRNP 适用: 人 WB 宿主: 小鼠 Monoclonal EM-20 unconjugated
anti-Prion Protein (PRNP) (AA 143-230) antibodyPRNP 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Western Blot: 1/2500. Immunohistochemistry: 1/1000-1/5000. Quality Control: Antibodies were analyzed by Immunocytochemistry (1/1000 dilution) using cells transfected with the prion protein cDNA.
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限制
- 仅限研究用
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缓冲液
- Buffer System: 0.9 % (w/v) Sodium Phosphate (10 mM, pH 7.2) buffered isotonic saline, 50 % (v/v) Glycerol, with 0.02 % (w/v) Sodium Azide as an anti-microbial agent
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Upon receipt, store undiluted (in aliquots) at -20°C. This products is photosensitive and should be protected from light. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
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- PRNP (Prion Protein (PRNP))
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别名
- cd230,prp
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背景
- Prion protein is the protein responsible for human Creutzfeldt-Jakob Disease (CJD), and somatic mutations of the prion gene are responsible for Gerstmann-Straussler-Scheinker disease and Fatal Familial Insomnia. The bovine homolog of the prion protein is responsible for bovine Spongioform Encephalopathy (BSE), also known as Mad Cow Disease. In all cases, the prion protein has two folding states, with one state being the normal non-pathological form, and the other state inducing a spongioform encephalopathy.
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UniProt
- P23907
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途径
- Transition Metal Ion Homeostasis, Activated T Cell Proliferation
抗原
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