Beta-Phosphoglucomutase 抗体 (Biotin)
This anti-Beta-Phosphoglucomutase antibody is a 山羊 多克隆 antibody detecting Beta-Phosphoglucomutase in WB 和 EIA. Suitable for Lactococcus lacti.
Quick Overview for Beta-Phosphoglucomutase 抗体 (Biotin) (ABIN5540026)
抗原
Beta-Phosphoglucomutase
(Phosphoglucomutase, beta-)
适用
Lactococcus lacti
宿主
山羊
克隆类型
多克隆
标记
This Beta-Phosphoglucomutase antibody is conjugated to Biotin
应用范围
Western Blotting (WB), Enzyme Immunoassay (EIA)
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特异性
- This antibody detects Beta-phosphoglucomutase from Lactococcus lacti. Cross reactivity against Beta-phosphoglucomutase from other sources is unknown. Immunoelectrophoresis give a single precipitin arc against anti-biotin, anti-goat serum as well as purified and partially purified beta-phosphoglucomutase [ Lactococcus lacti ].
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纯化方法
- Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
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免疫原
- Beta-phosphoglucomutase (PGMB) from Lactococcus lacti
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anti-Phosphoglucomutase, beta- antibody (Biotin)
适用: Lactococcus lacti WB, ELISA 宿主: 山羊 Polyclonal Biotin
anti-Phosphoglucomutase, beta- antibody适用: Lactococcus lacti WB, ELISA 宿主: 山羊 Polyclonal unconjugated
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应用备注
- Western blot : 1/500-1/3000. ELISA : 1/5,000-1/25,000 dilution suggested to begin optimization. This product has been assayed against 1.0 μg of Beta-phosphoglucomutase [ Lactococcus lacti ] in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/10,000 to 1/50,000 of the reconstitution concentration is suggested.
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限制
- 仅限研究用
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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有效期
- 12 months
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- Beta-Phosphoglucomutase (Phosphoglucomutase, beta-)
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别名
- pgmb
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UniProt
- P71447
抗原
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