This 22D3 Monoclonal antibody is highly specific for Alpha-Cardiac Actin, and does not cross react with other Actin isoforms.
纯化方法
Purified
免疫原
Peptide comprising the N-terminal nonapeptide of alpha-Cardiac Actin with an acetylated N-terminus coupled to keyhole limpet hemocyanin through the cysteine residue.
ELISA. Flow Cytometry. Immunoblotting. Immunofluorescence. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin-Embedded Sections. Recommended Dilutions : 1/100-1/1000 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent and 1/1000-1/5000 for immunoblotting applications.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C,-20 °C
储存方法
Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles. Shelf life: One year from despatch.
抗原
ACTC1
(Actin, Alpha, Cardiac Muscle 1 (ACTC1))
别名
alpha 1 cardiac muscle actin,actc1
背景
Among the six actin isoforms described in mammals, two are found in virtually all cells (β- and γ-cytoplasmic), two are detected in smooth muscle cells (α- and γ-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (α-skeletal) and one in cardiac (α-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus, but the sequence of each of these actins is highly conserved in higher vertebrates. Cardiac α-actin, which is the main actin isoform in the adult heart, has also been shown to be the predominant form in early muscle development. In later development the expression of α-cardiac actin is down regulated and α-skeletal actin becomes the dominant isoform in the adult skeletal muscle.