FGF14 抗体 (N-Term)
Quick Overview for FGF14 抗体 (N-Term) (ABIN5539608)
抗原
See all FGF14 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- N-Term
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原理
- Fgf14 (mouse N terminus)
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序列
- REQHWDRPSA SR
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特异性
- This antibody is expected to recognize mouse isoform a (NP_034331.2) and human isoform 1A (NP_004106.1).
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交叉反应
- Cow, 犬, 人, 小鼠, Pig, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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亚型
- IgG
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anti-Fibroblast Growth Factor 14 (FGF14) (AA 1-252) antibody
FGF14 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Fibroblast Growth Factor 14 (FGF14) (AA 207-241), (C-Term) antibodyFGF14 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal RB52181 unconjugated
anti-Fibroblast Growth Factor 14 (FGF14) (AA 138-247) antibodyFGF14 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Fibroblast Growth Factor 14 (FGF14) (N-Term) antibodyFGF14 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fibroblast Growth Factor 14 (FGF14) (AA 151-200) antibodyFGF14 适用: 人, 小鼠, 大鼠, Cow, 马, 豚鼠, 兔, 绵羊, 小鸡, Bat, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Fibroblast Growth Factor 14 (FGF14) antibodyFGF14 适用: 人, 小鼠, 大鼠 WB, IF, IC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry: Cryosection of immersion-fixed (1 % PFA/0.5 % methanol) Human Hypothalamus. Optimal dilution: 1:5000. *Vibratome prepared sample for optimal labelling.
Western Blot: Approx 28 kDa band observed in Mouse and Human Brain lysates (calculated MW of 28.3 kDa according to NP_997550.1 and 27.7 kDa according to NP_034331.2). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.
Peptide ELISA: antibody detection limit dilution 1:128000.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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