NEK2 抗体 (AA 396-426)
Our Local Distributor
北京 101111
Quick Overview for NEK2 抗体 (AA 396-426) (ABIN5537201)
抗原
See all NEK2 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 396-426
-
纯化方法
- This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
-
免疫原
- This NEK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 396-426 amino acids from the Central region of human NEK2.
-
亚型
- Ig Fraction
-
-
-
-
应用备注
-
For IHC-P starting dilution is: 1:100
For IF starting dilution is: 1:25
For WB starting dilution is: 1:1000 -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 2 mg/mL
-
缓冲液
- Supplied in PBS with 0.09 % (W/V) sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
-
-
- NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))
-
别名
- NEK2
-
背景
- Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
-
分子量
- 52 kDa
-
基因ID
- 4751
-
UniProt
- P51955
-
途径
- M Phase
抗原
-