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DKK3 抗体 (N-Term)

This anti-DKK3 antibody is a 兔多克隆 antibody detecting DKK3 in WB 和 IHC (p). Suitable for 人.

Western blot analysis of lysates from 293, 293T, A549, HL-60, MCF-7 cell line and mouse brain tissue lysate (from left to right), using DKK3 Antibody (A30) at 1:1000 at each lane. (DKK3 抗体 (N-Term))

Formalin-fixed and paraffin-embedded human brain tissue reacted with DKK3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (DKK3 抗体 (N-Term))

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with DKK3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (DKK3 抗体 (N-Term))

3 images

产品编号 ABIN5532899

发货至: 中国

Quick Overview for DKK3 抗体 (N-Term) (ABIN5532899)

抗原

See all DKK3 抗体

DKK3

适用

52
35
17
16
3
2
2
1
1
1
1

人

宿主

兔

克隆类型

多克隆

标记

39
5
5
4
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1

This DKK3 antibody is un-conjugated

应用范围

56
35
26
13
13
11
8
7
7
6
4
1
1

Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Discover our top product DKK3 Primary Antibody

抗原表位
15

9

8

7

4

2

2

2

1

1

1

1

1

1

1

1

1

1
AA 15-45, N-Term

纯化方法

This antibody is purified through a protein A column, followed by peptide affinity purification.

免疫原

This DKK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-45 amino acids from the N-terminal region of human DKK3.

亚型

Ig Fraction
anti-DKK3 (AA 23-349) antibody
适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-DKK3 (AA 91-350) antibody
适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 8A5C6 unconjugated

anti-DKK3 (AA 91-350) antibody
适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal unconjugated

anti-DKK3 (AA 31-130) antibody
适用: 小鼠, 兔 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-DKK3 (AA 22-350) antibody
适用: 人 WB, ELISA, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-DKK3 (AA 22-350) antibody
适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-DKK3 antibody
适用: 人, 猴 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 4G7 unconjugated

anti-DKK3 (AA 41-348) antibody
适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-DKK3 (C-Term) antibody
Verified 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated

anti-DKK3 antibody
适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated

应用备注

For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

限制

仅限研究用
状态

Liquid

浓度

0.45 mg/mL

缓冲液

Supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
抗原

DKK3

背景

DKK3, like DKK1, DKK2, and DKK4, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed wide expression of the DKK3 transcript, with highest expression in heart, brain, and spinal cord. In situ hybridization reveals highest expression in mouse brain, eye, and heart.

分子量

38 kDa

基因ID

27122

UniProt

Q9UBP4

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