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DKK3 抗体 (N-Term)

Name: DKK3 抗体 (N-Term)
SKU: ABIN5532899
Availability: OutOfStock

适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

Western blot analysis of lysates from 293, 293T, A549, HL-60, MCF-7 cell line and mouse brain tissue lysate (from left to right), using DKK3 Antibody (A30) at 1:1000 at each lane. (DKK3 抗体 (N-Term))

Formalin-fixed and paraffin-embedded human brain tissue reacted with DKK3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (DKK3 抗体 (N-Term))

IHC

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with DKK3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (DKK3 抗体 (N-Term))

IHC

3 images

产品编号 ABIN5532899

发货至: 中国

Datasheet as PDF

抗原 See all DKK3 抗体

DKK3
抗原表位
15

8

8

7

4

2

1

1

1

1

1

1

1

1

1

1

1

1
AA 15-45, N-Term
适用
50

33

17

13

2

2

2

1

1

1

1
人
宿主
61

7

1

1
兔
Compatible Secondaries
克隆类型
60

10
多克隆
标记
34

5

5

4

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This DKK3 antibody is un-conjugated
应用范围
51

34

23

13

13

6

6

6

5

4

4

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

纯化方法

This antibody is purified through a protein A column, followed by peptide affinity purification.

免疫原

This DKK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-45 amino acids from the N-terminal region of human DKK3.

亚型

Ig Fraction

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anti-DKK3 (AA 31-130) antibody
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适用: 人 WB, ELISA, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-DKK3 (AA 22-350) antibody
适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

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适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 4G7 unconjugated

anti-DKK3 (AA 41-348) antibody
适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-DKK3 (C-Term) antibody
Verified 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated

anti-DKK3 antibody
适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated

应用备注

For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

限制

仅限研究用
状态

Liquid

浓度

0.45 mg/mL

缓冲液

Supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
抗原

DKK3

Abstract

DKK3 产品

背景

DKK3, like DKK1, DKK2, and DKK4, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed wide expression of the DKK3 transcript, with highest expression in heart, brain, and spinal cord. In situ hybridization reveals highest expression in mouse brain, eye, and heart.

分子量

38 kDa

基因ID

27122

UniProt

Q9UBP4