MMP16 抗体 (AA 120-296)
(2 references)Quick Overview for MMP16 抗体 (AA 120-296) (ABIN5519039)
抗原
See all MMP16 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 120-296
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原理
- Anti-MMP16 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-MMP16 Antibody Picoband® (ABIN5519039). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human MMP16 recombinant protein (Position: Y120-I296).
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亚型
- IgG
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anti-Matrix Metallopeptidase 16 (Membrane-inserted) (MMP16) (AA 290-565) antibody
MMP16 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Matrix Metallopeptidase 16 (Membrane-inserted) (MMP16) (C-Term) antibodyMMP16 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Matrix Metallopeptidase 16 (Membrane-inserted) (MMP16) (AA 501-607) antibodyMMP16 适用: 人, 小鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Matrix Metallopeptidase 16 (Membrane-inserted) (MMP16) (AA 519-548), (C-Term) antibodyMMP16 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB33657 unconjugated
anti-Matrix Metallopeptidase 16 (Membrane-inserted) (MMP16) (C-Term) antibodyMMP16 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Matrix Metallopeptidase 16 (Membrane-inserted) (MMP16) antibodyMMP16 适用: 人, 小鼠, 大鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Matrix Metallopeptidase 16 (Membrane-inserted) (MMP16) (C-Term) antibodyMMP16 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Matrix Metallopeptidase 16 (Membrane-inserted) (MMP16) (AA 72-121) antibodyMMP16 适用: 人, 小鼠, 大鼠, Cow, 犬, Pig, 小鸡, 兔, 猴, 马, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Matrix Metallopeptidase 16 (Membrane-inserted) (MMP16) (AA 351-557) antibodyMMP16 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Matrix Metallopeptidase 16 (Membrane-inserted) (MMP16) (AA 154-183), (N-Term) antibodyMMP16 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB2031 unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
ELISA, 0.1-0.5 μg/mL
1. Sato, H., Tanaka, M., Takino, T., Inoue, M., Seiki, M. : Assignment of the human genes for membrane-type-1, -2, and -3 matrix metalloproteinases (MMP14, MMP15, and MMP16) to 14q12.2, 16q12.2-q21, and 8q21, respectively, by in situ hybridization. Genomics 39: 412-413, 1997. 2. Takino, T., Sato, H., Shinagawa, A., Seiki, M. : Identification of the second membrane-type matrix metalloproteinase (MT-MMP-2) gene from a human placenta cDNA library: MT-MMPs form a unique membrane-type subclass in the MMP family. J. Biol. Chem. 270: 23013-23020, 1995. -
说明
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We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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: "MT3-MMP down-regulation promotes tumorigenesis and correlates to poor prognosis in esophageal squamous cell carcinoma." in: Cancer medicine, Vol. 5, Issue 9, pp. 2459-68, (2017) (PubMed).
: "miR-132 can inhibit glioma cells invasion and migration by target MMP16 in vitro." in: OncoTargets and therapy, Vol. 8, pp. 3211-8, (2015) (PubMed).
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: "MT3-MMP down-regulation promotes tumorigenesis and correlates to poor prognosis in esophageal squamous cell carcinoma." in: Cancer medicine, Vol. 5, Issue 9, pp. 2459-68, (2017) (PubMed).
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- MMP16 (Matrix Metallopeptidase 16 (Membrane-inserted) (MMP16))
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别名
- MMP16
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背景
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Synonyms: Matrix metalloproteinase-16, MMP-16, 3.4.24.-, MMP-X2, Membrane-type matrix metalloproteinase 3, MT-MMP 3, MTMMP3, Membrane-type-3 matrix metalloproteinase, MT3-MMP, MT3MMP, MMP16, MMPX2,
Tissue Specificity: Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary.
Background: The matrix metalloproteinase 16 (MMP16) protein consists of 604 amino acids and has a characteristic MMP domain structure, which gene is mapped on human chromosome 8q21. Additionally, MMP16 has a C-terminal extension containing a potential transmembrane domain, similar to MMP14, MMP15, and MMP17. Furthermore, it is membrane-bound and is a member of the membrane-type MMPs that are a subclass in the MMP family since the other members lack a C-terminal transmembrane domain and are secreted as soluble forms. MMP16 is expressed as a 12-kb transcript in brain, placenta, heart, and some carcinoma cell lines, but is not detectably expressed in lung, kidney, liver, spleen, and muscle.
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分子量
- 53 kDa
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基因ID
- 4325
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UniProt
- P51512
抗原
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