MED20 抗体 (AA 1-212)
Quick Overview for MED20 抗体 (AA 1-212) (ABIN5519037)
抗原
See all MED20 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-212
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原理
- Anti-TRFP/MED20 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-TRFP/MED20 Antibody Picoband® (ABIN5519037). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human TRFP / MED20 recombinant protein (Position: M1-R212).
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亚型
- IgG
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anti-Mediator Complex Subunit 20 (MED20) (AA 1-212) antibody
MED20 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Mediator Complex Subunit 20 (MED20) (AA 1-212) antibodyMED20 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 20 (MED20) (Middle Region) antibodyMED20 适用: 人, 大鼠, 小鼠, Cow, 犬, 山羊, 豚鼠, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Sato, S., Tomomori-Sato, C., Banks, C. A. S., Parmely, T. J., Sorokina, I., Brower, C. S., Conaway, R. C., Conaway, J. W. A mammalian homolog of Drosophila melanogaster transcriptional coactivator Intersex is a subunit of the mammalian Mediator complex. J. Biol. Chem. 278: 49671-49674, 2003. 2. Xiao, H., Tao, Y., Roeder, R. G. The human homologue of Drosophila TRF-proximal protein is associated with an RNA polymerase II-SRB complex. J. Biol. Chem. 274: 3937-3940, 1999. -
说明
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We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- MED20 (Mediator Complex Subunit 20 (MED20))
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别名
- MED20
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背景
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Synonyms: Mediator of RNA polymerase II transcription subunit 20, Mediator complex subunit 20, TRF-proximal protein homolog, hTRFP, MED20, TRFP,
Tissue Specificity: Pre-B-cells and B-cells but not terminally differentiated plasma cells.
Background: MED20 is a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. A mutation in this gene has been associated with a novel infantile-onset neurodegenerative movement disorder.
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分子量
- 23 kDa
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基因ID
- 9477
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UniProt
- Q9H944
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途径
- Regulation of Lipid Metabolism by PPARalpha
抗原
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