MED18 抗体 (AA 1-208)
Quick Overview for MED18 抗体 (AA 1-208) (ABIN5519036)
抗原
See all MED18 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 1-208
-
原理
- Anti-MED18 Antibody
-
交叉反应 （详细）
- No cross-reactivity with other proteins.
-
产品特性
- Anti-MED18 Antibody (ABIN5519036). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- E. coli-derived human MED18 recombinant protein (Position: M1-M208).
-
亚型
- IgG
-
-
anti-Mediator Complex Subunit 18 (MED18) (AA 35-208) antibody
MED18 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 18 (MED18) (AA 1-208) antibodyMED18 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 18 (MED18) (AA 30-110) antibodyMED18 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 18 (MED18) (Internal Region) antibodyMED18 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 18 (MED18) (AA 1-208) antibodyMED18 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Mediator Complex Subunit 18 (MED18) (AA 82-131) antibodyMED18 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼, Hamster, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
-
说明
-
We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
-
-
- MED18 (Mediator Complex Subunit 18 (MED18))
-
别名
- MED18
-
背景
-
Synonyms: Mediator of RNA polymerase II transcription subunit 18, Mediator complex subunit 18, p28b, MED18,
Tissue Specificity: Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.
Background: MED18 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II. Using in vitro-translated epitope-tagged proteins for protein-binding assays, it is found that MED18 ly interacted with the Mediator subunit TRFP (MED20). The MED18-TRFP heterodimer could also be coimmunoprecipitated from cotransfected insect cells. The MED18 gene was mapped to chromosome 1p35.3 based on an alignment of the MED18 sequence with the genomic sequence.
-
分子量
- 24 kDa
-
基因ID
- 54797
-
途径
- Regulation of Lipid Metabolism by PPARalpha
抗原
-
