MED18 抗体 (AA 1-208)
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- 抗原 See all MED18 抗体
- MED18 (Mediator Complex Subunit 18 (MED18))
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抗原表位
- AA 1-208
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This MED18 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
- 原理
- Anti-MED18 Antibody Picoband®
- 交叉反应 （详细）
- No cross-reactivity with other proteins.
- 产品特性
- Anti-MED18 Antibody Picoband® (ABIN5519036). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- 纯化方法
- Immunogen affinity purified.
- 免疫原
- E. coli-derived human MED18 recombinant protein (Position: M1-M208).
- 亚型
- IgG
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anti-Mediator Complex Subunit 18 (MED18) (AA 35-208) antibody
MED18 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 18 (MED18) (AA 1-208) antibodyMED18 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 18 (MED18) (Internal Region) antibodyMED18 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 18 (MED18) (AA 1-208) antibodyMED18 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Mediator Complex Subunit 18 (MED18) (AA 30-110) antibodyMED18 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hartz, P. A. Personal Communication. Baltimore, Md. 10/30/2008. 2. Sato, S., Tomomori-Sato, C., Banks, C. A. S., Sorokina, I., Parmely, T. J., Kong, S. E., Jin, J., Cai, Y., Lane, W. S., Brower, C. S., Conaway, R. C., Conaway, J. W. Identification of mammalian mediator subunits with similarities to yeast mediator subunits Srb5, Srb6, Med11, and Rox3. J. Biol. Chem. 278: 15123-15127, 2003. - 说明
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We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- 抗原
- MED18 (Mediator Complex Subunit 18 (MED18))
- 别名
- MED18 (MED18 产品)
- 背景
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Synonyms: Mediator of RNA polymerase II transcription subunit 18, Mediator complex subunit 18, p28b, MED18,
Tissue Specificity: Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.
Background: MED18 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II. Using in vitro-translated epitope-tagged proteins for protein-binding assays, it is found that MED18 ly interacted with the Mediator subunit TRFP (MED20). The MED18-TRFP heterodimer could also be coimmunoprecipitated from cotransfected insect cells. The MED18 gene was mapped to chromosome 1p35.3 based on an alignment of the MED18 sequence with the genomic sequence.
- 分子量
- 24 kDa
- 基因ID
- 54797
- 途径
- Regulation of Lipid Metabolism by PPARalpha
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