MED15 抗体 (AA 1-285)
Quick Overview for MED15 抗体 (AA 1-285) (ABIN5519035)
抗原
See all MED15 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-285
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原理
- Anti-MED15 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-MED15 Antibody Picoband® (ABIN5519035). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human MED15 recombinant protein (Position: M1-A285).
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亚型
- IgG
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anti-Mediator Complex Subunit 15 (MED15) (AA 1-88) antibody
MED15 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4A4 unconjugated
anti-Mediator Complex Subunit 15 (MED15) (AA 712-746), (C-Term) antibodyMED15 适用: 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB51171 unconjugated
anti-Mediator Complex Subunit 15 (MED15) (AA 433-530) antibodyMED15 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 15 (MED15) (C-Term) antibodyMED15 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 15 (MED15) (C-Term) antibodyMED15 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 15 (MED15) antibodyMED15 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 15 (MED15) (AA 743-779), (C-Term) antibodyMED15 适用: 人, 大鼠 WB 宿主: 兔 Polyclonal RB51972 unconjugated
anti-Mediator Complex Subunit 15 (MED15) (Internal Region) antibodyVerified MED15 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Fassler JS, Winston F (Dec 1989). "The Saccharomyces cerevisiae SPT13/GAL11 gene has both positive and negative regulatory roles in transcription". Molecular and Cellular Biology. 9 (12): 5602-9. 2. Suzuki Y, Nogi Y, Abe A, Fukasawa T (Nov 1988). "GAL11 protein, an auxiliary transcription activator for genes encoding galactose-metabolizing enzymes in Saccharomyces cerevisiae". Molecular and Cellular Biology. 8 (11): 4991-9. -
说明
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We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- MED15 (Mediator Complex Subunit 15 (MED15))
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别名
- MED15
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背景
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Synonyms: Mediator of RNA polymerase II transcription subunit 15, Activator-recruited cofactor 105 kDa component, ARC105, CTG repeat protein 7a, Mediator complex subunit 15, Positive cofactor 2 glutamine/Q-rich-associated protein, PC2 glutamine/Q-rich-associated protein, TPA-inducible gene 1 protein, TIG-1, Trinucleotide repeat-containing gene 7 protein, MED15, ARC105, CTG7A, PCQAP, TIG1, TNRC7,
Tissue Specificity: Expressed in all tissues examined, including heart, brain, lung, spleen, thymus, pancreas, blood leukocyte and placenta. However, the level of expression varied, with highest expression in the placenta and peripheral blood and lowest in the pancreas and kidney.
Background: Mediator of RNA polymerase II transcription subunit 15, also known as Gal11, Spt13 in yeast and PCQAP, ARC105, or TIG-1 in humans is a protein encoded by the MED15 gene. The protein encoded by this gene is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. This gene contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome.
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分子量
- 97 kDa
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基因ID
- 51586
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途径
- Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha
抗原
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