MED13 抗体 (AA 61-240)
Quick Overview for MED13 抗体 (AA 61-240) (ABIN5519033)
抗原
See all MED13 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 61-240
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原理
- Anti-MED13 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-MED13 Antibody Picoband® (ABIN5519033). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human MED13 recombinant protein (Position: L61-Y240).
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亚型
- IgG
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anti-Mediator Complex Subunit 13 (MED13) antibody
MED13 适用: 人 ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 13 (MED13) (AA 681-730) antibodyMED13 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
ELISA, 0.1-0.5 μg/mL
1. Gustafsson CM, Samuelsson T (Jul 2001). "Mediator--a universal complex in transcriptional regulation". Molecular Microbiology. 41 (1): 1-8. 2. Tuchin DA, Erve PR, Schumer W (1976). "Prevention of endotoxin--induced irreversible platelet aggregation in vitro". Surgical Forum. 27 (62): 22-3. -
说明
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We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- MED13 (Mediator Complex Subunit 13 (MED13))
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别名
- MED13
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背景
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Synonyms: Mediator of RNA polymerase II transcription subunit 13, Activator-recruited cofactor 250 kDa component, ARC250, Mediator complex subunit 13, Thyroid hormone receptor-associated protein 1, Thyroid hormone receptor-associated protein complex 240 kDa component, Trap240, Vitamin D3 receptor-interacting protein complex component DRIP250, DRIP250, MED13, ARC250, KIAA0593, THRAP1, TRAP240,
Tissue Specificity: Ubiquitous.
Background: Mediator complex subunit 13 is a protein that in humans is encoded by the MED13 gene. This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. The product of this gene is proposed to form a sub-complex with MED12, cyclin C, and CDK8 that can negatively regulate transactivation by mediator.
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分子量
- 300 kDa
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基因ID
- 9969
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途径
- Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Regulation of Lipid Metabolism by PPARalpha
抗原
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