MED9 抗体 (AA 55-146)
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- 抗原 See all MED9 抗体
- MED9 (Mediator Complex Subunit 9 (MED9))
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抗原表位
- AA 55-146
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This MED9 antibody is un-conjugated
- 应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
- 原理
- Anti-MED9 Antibody Picoband®
- 交叉反应 （详细）
- No cross-reactivity with other proteins.
- 产品特性
- Anti-MED9 Antibody Picoband® (ABIN5519032). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- 纯化方法
- Immunogen affinity purified.
- 免疫原
- E. coli-derived human MED9 recombinant protein (Position: A55-E146).
- 亚型
- IgG
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anti-Mediator Complex Subunit 9 (MED9) (AA 1-146) antibody
MED9 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 7F1 unconjugated
anti-Mediator Complex Subunit 9 (MED9) (AA 1-146) antibodyMED9 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
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- 应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
ELISA, 0.1-0.5 μg/mL
1. Tomomori-Sato, C., Sato, S., Parmely, T. J., Banks, C. A. S., Sorokina, I., Florens, L., Zybailov, B., Washburn, M. P., Brower, C. S., Conaway, R. C., Conaway, J. W. A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2. J. Biol. Chem. 279: 5846-5851, 2004. - 说明
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We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- 抗原
- MED9 (Mediator Complex Subunit 9 (MED9))
- 别名
- MED9 (MED9 产品)
- 背景
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Synonyms: Mediator of RNA polymerase II transcription subunit 9, Mediator complex subunit 9, MED9, MED25,
Tissue Specificity: Expressed in fetal brain, fetal lung, fetal liver, heart, brain, placenta, lung, liver, muscle, kidney and pancreas.
Background: MED9 is a member of the mediator complex of proteins that promote activation of RNA polymerase II through interactions with transcription factors. This MED9gene is located within the Smith-Magenis syndrome region on chromosome 17.
- 分子量
- 19 kDa
- 基因ID
- 55090
- 途径
- Regulation of Lipid Metabolism by PPARalpha
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