MED9 抗体 (AA 55-146)
Quick Overview for MED9 抗体 (AA 55-146) (ABIN5519032)
抗原
See all MED9 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 55-146
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原理
- Anti-MED9 Antibody Picoband®
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交叉反应 (详细)
- No cross-reactivity with other proteins.
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产品特性
- Anti-MED9 Antibody Picoband® (ABIN5519032). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human MED9 recombinant protein (Position: A55-E146).
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亚型
- IgG
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
ELISA, 0.1-0.5 μg/mL
1. Tomomori-Sato, C., Sato, S., Parmely, T. J., Banks, C. A. S., Sorokina, I., Florens, L., Zybailov, B., Washburn, M. P., Brower, C. S., Conaway, R. C., Conaway, J. W. A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2. J. Biol. Chem. 279: 5846-5851, 2004. -
说明
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We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- MED9 (Mediator Complex Subunit 9 (MED9))
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别名
- MED9
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背景
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Synonyms: Mediator of RNA polymerase II transcription subunit 9, Mediator complex subunit 9, MED9, MED25,
Tissue Specificity: Expressed in fetal brain, fetal lung, fetal liver, heart, brain, placenta, lung, liver, muscle, kidney and pancreas.
Background: MED9 is a member of the mediator complex of proteins that promote activation of RNA polymerase II through interactions with transcription factors. This MED9gene is located within the Smith-Magenis syndrome region on chromosome 17.
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分子量
- 19 kDa
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基因ID
- 55090
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途径
- Regulation of Lipid Metabolism by PPARalpha
抗原
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