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MED8 抗体 (AA 1-195)

MED8 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC, IHC, FACS 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN5519031
发货至: 中国
  • 抗原 See all MED8 抗体
    MED8 (Mediator Complex Subunit 8 (MED8))
    抗原表位
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 1-195
    适用
    • 29
    • 13
    • 12
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    人, 小鼠, 大鼠
    宿主
    • 24
    • 5
    克隆类型
    • 25
    • 4
    多克隆
    标记
    • 17
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This MED8 antibody is un-conjugated
    应用范围
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FACS)
    原理
    Anti-MED8 Antibody Picoband®
    交叉反应 (详细)
    No cross-reactivity with other proteins.
    产品特性
    Anti-MED8 Antibody Picoband® (ABIN5519031). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    纯化方法
    Immunogen affinity purified.
    免疫原
    E. coli-derived human MED8 recombinant protein (Position: M1-R195).
    亚型
    IgG
    Top Product
    Discover our top product MED8 Primary Antibody
  • 应用备注
    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat
    Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    ELISA, 0.1-0.5 μg/mL
    1. Brower CS, Sato S, Tomomori-Sato C, Kamura T, Pause A, Stearman R, Klausner RD, Malik S, Lane WS, Sorokina I, Roeder RG, Conaway JW, Conaway RC (Aug 2002). "Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase". ProcNatlAcadSci U S A. 99 (16): 10353-8. 2. Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC (Jun 2004). "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology". Mol. Cell. 14 (5): 685-91.
    说明

    We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    浓度
    500 μg/mL
    缓冲液
    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • 抗原
    MED8 (Mediator Complex Subunit 8 (MED8))
    别名
    MED8 (MED8 产品)
    背景

    Synonyms: Mediator of RNA polymerase II transcription subunit 8, Activator-recruited cofactor 32 kDa component, ARC32, Mediator complex subunit 8, MED8,

    Tissue Specificity: Ubiquitous.

    Background: Mediator of RNA polymerase II transcription subunit 8 is an enzyme that in humans is encoded by the MED8 gene. This gene encodes a protein component of the mediator complex, which aids in transcriptional activation through interaction with RNA polymerase II and gene-specific transcription factors. The encoded protein may also function in ubiquitin ligation and protein degradation.

    分子量
    29 kDa
    基因ID
    112950
    UniProt
    Q96G25
    途径
    Regulation of Lipid Metabolism by PPARalpha
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