MED8 抗体 (AA 1-195)
Quick Overview for MED8 抗体 (AA 1-195) (ABIN5519031)
抗原
See all MED8 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-195
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原理
- Anti-MED8 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-MED8 Antibody Picoband® (ABIN5519031). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human MED8 recombinant protein (Position: M1-R195).
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亚型
- IgG
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anti-Mediator Complex Subunit 8 (MED8) antibody
MED8 适用: 人, 小鼠, 大鼠 WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 8 (MED8) (AA 1-59) antibodyMED8 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1D3 unconjugated
anti-Mediator Complex Subunit 8 (MED8) (C-Term) antibodyMED8 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 8 (MED8) (Internal Region) antibodyMED8 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 8 (MED8) (AA 1-59) antibodyMED8 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Mediator Complex Subunit 8 (MED8) (Internal Region) antibodyMED8 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 8 (MED8) (AA 1-59) antibodyMED8 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1E5 unconjugated
anti-Mediator Complex Subunit 8 (MED8) (N-Term) antibodyMED8 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 斑马鱼, Bat, Hamster, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 8 (MED8) (N-Term) antibodyMED8 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 8 (MED8) (Internal Region) antibodyMED8 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL
1. Brower CS, Sato S, Tomomori-Sato C, Kamura T, Pause A, Stearman R, Klausner RD, Malik S, Lane WS, Sorokina I, Roeder RG, Conaway JW, Conaway RC (Aug 2002). "Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase". ProcNatlAcadSci U S A. 99 (16): 10353-8. 2. Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC (Jun 2004). "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology". Mol. Cell. 14 (5): 685-91. -
说明
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We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- MED8 (Mediator Complex Subunit 8 (MED8))
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别名
- MED8
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背景
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Synonyms: Mediator of RNA polymerase II transcription subunit 8, Activator-recruited cofactor 32 kDa component, ARC32, Mediator complex subunit 8, MED8,
Tissue Specificity: Ubiquitous.
Background: Mediator of RNA polymerase II transcription subunit 8 is an enzyme that in humans is encoded by the MED8 gene. This gene encodes a protein component of the mediator complex, which aids in transcriptional activation through interaction with RNA polymerase II and gene-specific transcription factors. The encoded protein may also function in ubiquitin ligation and protein degradation.
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分子量
- 29 kDa
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基因ID
- 112950
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UniProt
- Q96G25
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途径
- Regulation of Lipid Metabolism by PPARalpha
抗原
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