FH 抗体
Quick Overview for FH 抗体 (ABIN5518986)
抗原
See all FH 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-FH Antibody Picoband®
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序列
- YDKAAKIAKT AHKNGSTLKE TAIELGYLTA EQFDEWVKPK DMLGPK
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
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Rabbit IgG polyclonal antibody for FH detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: fumarate hydratase
Protein Name: Fumarate hydratase, mitochondrial -
纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human FH, which shares 100% and 97.8% amino acid (aa) sequence identity with mouse and rat FH, respectively.
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亚型
- IgG
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anti-Fumarate Hydratase (FH) (AA 45-188) antibody
FH 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fumarate Hydratase (FH) antibodyFH 适用: 人, 大鼠, 犬 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 1F10 unconjugated
anti-Fumarate Hydratase (FH) antibodyKO Validated FH 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Fumarate Hydratase (FH) (Internal Region) antibodyVerified FH 适用: 人 WB, ELISA, IHC, EIA 宿主: 山羊 Polyclonal unconjugated
anti-Fumarate Hydratase (FH) (AA 33-510) antibodyFH 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 5C12 unconjugated
anti-Fumarate Hydratase (FH) (AA 33-510) antibodyFH 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3E7 unconjugated
anti-Fumarate Hydratase (FH) (AA 33-510) antibodyFH 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2E10 unconjugated
anti-Fumarate Hydratase (FH) (AA 42-185) antibodyFH 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Fumarate Hydratase (FH) (AA 33-510) antibodyFH 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5D4 unconjugated
anti-Fumarate Hydratase (FH) (AA 33-510) antibodyFH 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2C5 unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells1. Begley TP, McMurry J (2005). The organic chemistry of biological pathways. Roberts and Co. Publishers. 2. Weaver T (October 2005). "Structure of free fumarase C from Escherichia coli". ActaCrystallogr. D. 61 (Pt 10): 1395-401. -
说明
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We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- FH (Fumarate Hydratase (FH))
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别名
- FH
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背景
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Synonyms: Fumarate hydratase, mitochondrial, Fumarase, 4.2.1.2, FH,
Tissue Specificity: Expressed in red blood cells, underexpressed in red blood cells (cytoplasm) of patients with hereditary non- spherocytic hemolytic anemia of unknown etiology.
Background: Fumarase (or fumaratehydratase) is an enzyme that catalyzes the reversible hydration/dehydration of fumarate to malate. Fumarase comes in two forms: mitochondrial and cytosolic. The mitochondrial isoenzyme is involved in the Krebs Cycle (also known as the Tricarboxylic Acid Cycle [TCA] or the Citric Acid Cycle), and the cytosolic isoenzyme is involved in the metabolism of amino acids and fumarate. Subcellular localization is established by the presence of a signal sequence on the amino terminus in the mitochondrial form, while subcellular localization in the cytosolic form is established by the absence of the signal sequence found in the mitochondrial variety. This enzyme participates in 2 metabolic pathways: citric acid cycle, reductive citric acid cycle (CO2 fixation), and is also important in renal cell carcinoma. Mutations in this gene have been associated with the development of leiomyomas in the skin and uterus in combination with renal cell carcinoma.
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分子量
- 49 kDa
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基因ID
- 2271
抗原
-
