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RAN 抗体 (AA 2-216)

This anti-RAN antibody is a 兔 多克隆 antibody detecting RAN in WB, IHC, FACS, IF, IP 和 ICC. Suitable for 人, 小鼠 和 大鼠.
产品编号 ABIN5518950
发货至: 中国

Quick Overview for RAN 抗体 (AA 2-216) (ABIN5518950)

抗原

See all RAN 抗体
RAN (RAN, Member RAS Oncogene Family (RAN))

适用

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人, 小鼠, 大鼠

宿主

  • 79
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克隆类型

  • 76
  • 9
多克隆

标记

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This RAN antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)
  • 抗原表位

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    AA 2-216

    原理

    Anti-Ran Antibody Picoband®

    交叉反应 (详细)

    No cross-reactivity with other proteins.

    产品特性

    Anti-Ran Antibody Picoband® (ABIN5518950). Tested in Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    纯化方法

    Immunogen affinity purified.

    免疫原

    E. coli-derived human Ran recombinant protein (Position: A2-L216). Human Ran shares 100% amino acid (aa) sequence identity with both mouse and rat Ran.

    亚型

    IgG
  • 应用备注

    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat,
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
    Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
    Immunoprecipitation, 0.5-2 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Avis JM, Clarke PR (October 1996). "Ran, a GTPase involved in nuclear processes: its regulators and effectors". J. Cell. Sci. 109 (10): 2423-7. 2. Ciciarello M, Mangiacasale R, Lavia P (August 2007). "Spatial control of mitosis by the GTPase Ran". Cell. Mol. Life Sci. 64 (15): 1891-914. 3. Moore MS, Blobel G (May 1994). "A G protein involved in nucleocytoplasmic transport: the role of Ran". Trends Biochem. Sci. 19 (5): 211-6.

    说明

    We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • 抗原

    RAN (RAN, Member RAS Oncogene Family (RAN))

    别名

    RAN

    背景

    Synonyms: GTP-binding nuclear protein Ran,Androgen receptor-associated protein 24,GTPase Ran,Ras-like protein TC4,Ras-related nuclear protein,RAN,ARA24,OK/SW-cl.81,

    Tissue Specificity: Expressed in a variety of tissues. .

    Background: RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease.

    分子量

    24 kDa

    基因ID

    5901

    UniProt

    P62826

    途径

    Regulatory RNA Pathways, Intracellular Steroid Hormone Receptor Signaling Pathway, Protein targeting to Nucleus
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