Phenylalanine Hydroxylase 抗体 (AA 71-208)
Quick Overview for Phenylalanine Hydroxylase 抗体 (AA 71-208) (ABIN5518942)
抗原
See all Phenylalanine Hydroxylase 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 71-208
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原理
- Anti-PAH Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-PAH Antibody Picoband® (ABIN5518942). Tested in Flow Cytometry(Intracellular), IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human PAH recombinant protein (Position: R71-H208). Human PAH shares 89.1% and 88.4% amino acid (aa) sequence identity with mouse and rat PAH, respectively.
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亚型
- IgG
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anti-Phenylalanine Hydroxylase (AA 228-452) antibody
适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Phenylalanine Hydroxylase (Internal Region) antibodyVerified 适用: 人, 大鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Phenylalanine Hydroxylase (AA 1-226) antibody适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Phenylalanine Hydroxylase (AA 127-161) antibody适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 小鼠 Monoclonal 717CT21-1-1 unconjugated
anti-Phenylalanine Hydroxylase (AA 2-452) antibody适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Phenylalanine Hydroxylase (AA 1-452) antibody适用: 人 WB, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Phenylalanine Hydroxylase antibody适用: 人, 小鼠, 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Phenylalanine Hydroxylase (N-Term) antibody适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Phenylalanine Hydroxylase (AA 1-290) antibody适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Phenylalanine Hydroxylase (C-Term) antibody适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat,
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Fitzpatrick PF (1999). "Tetrahydropterin-dependent amino acid hydroxylases". Annual Review of Biochemistry. 68: 355-81. 2. Kaufman S (Feb 1958). "A new cofactor required for the enzymatic conversion of phenylalanine to tyrosine". The Journal of Biological Chemistry. 230 (2): 931-9. -
说明
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We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- Phenylalanine Hydroxylase
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别名
- PAH
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背景
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Synonyms: Phenylalanine-4-hydroxylase,PAH,1.14.16.1,Phe-4-monooxygenase,PAH,
Tissue Specificity: Predominantly expressed in lung and at lower level in kidney. Expressed in macrophages but not in vascular smooth muscle cells.
Background: Phenylalanine hydroxylase (PAH) is an enzyme that catalyzes the hydroxylation of the aromatic side-chain of phenylalanine to generate tyrosine. It is one of three members of the biopterin-dependent aromatic amino acid hydroxylases, a class of monooxygenase that uses tetrahydrobiopterin (BH4, a pteridine cofactor) and a non-heme iron for catalysis. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.
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分子量
- 45 kDa
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基因ID
- 5053
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UniProt
- P00439
抗原
-
