PPIA 抗体 (AA 116-165)
Quick Overview for PPIA 抗体 (AA 116-165) (ABIN5518864)
抗原
See all PPIA 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 116-165
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原理
- Anti-Cyclophilin A/PPIA Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Cyclophilin A/PPIA Antibody Picoband® (ABIN5518864). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human Cyclophilin A recombinant protein (Position: T116-E165). Human Cyclophilin A shares 98% and 95.9% amino acid (aa) sequence identity with mouse and rat Cyclophilin A, respectively.
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亚型
- IgG
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anti-Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA) (AA 2-165) antibody
PPIA 适用: 人 WB, IHC, ICC, IP, FACS 宿主: 兔 Polyclonal unconjugated
anti-Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA) (AA 2-164) antibodyPPIA 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA) (AA 2-164) antibodyPPIA 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA) (AA 1-165) antibodyPPIA 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1F4-1B5 unconjugated
anti-Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA) (C-Term) antibodyKO Validated PPIA 适用: 人 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA) (AA 2-165) antibodyPPIA 适用: 人 WB, IHC, ICC, IF 宿主: 小鼠 Monoclonal C10 unconjugated
anti-Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA) (AA 2-165) antibodyPPIA 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA) (AA 8-37), (N-Term) antibodyPPIA 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB20884 unconjugated
anti-Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA) (AA 101-165) antibodyPPIA 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA) (C-Term) antibodyPPIA 适用: 人, 小鼠, 大鼠, Cow, Pig, 猴, 犬, 山羊, 豚鼠, 马, 兔, Hamster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Luban, J., Bossolt, K. L., Franke, E. K., Kalpana, G. V., Goff, S. P.Human immunodeficiency virus type 1 gag protein binds to Cyclophilin A and B.Cell 73: 1067-1078, 1993. 2. Manel, N., Hogstad, B., Wang, Y., Levy, D. E., Unutmaz, D., Littman, D. R.A cryptic sensor for HIV-1 activates antiviral innate immunity in dendritic cells.Nature 467: 214-217, 2010. Note: Erratum: Nature 470: 424 only, 2011. 3. Willenbrink, W., Halaschek, J., Schuffenhauer, S., Kunz, J., Steinkasserer, A. Cyclophilin A, the major intracellular receptor for the immunosuppressant cyclosporin A, maps to chromosome 7p11.2-p13: four pseudogenes map to chromosomes 3, 10, 14, and 18.Genomics 28: 101-104, 1995. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- PPIA (Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA))
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别名
- PPIA
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背景
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Synonyms: Peptidyl-prolyl cis-trans isomerase A,PPIase A,5.2.1.8,Cyclophilin A,Cyclosporin A-binding protein,Rotamase A,Peptidyl-prolyl cis-trans isomerase A, N-terminally processed,PPIA,CYPA,
Tissue Specificity: Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.
Background: Cyclophilin A (PPIA), Peptidylprolyl isomerase A, is an enzyme that in humans is encoded by the PPIA gene. Using chromosome 7 and chromosome 10 deletion hybrid panels, the PPIA coding gene is localized to 7p13-p11.2. This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. Cyclophilin A is also a member of the immunophilin class of proteins that all possess peptidyl-prolyl cis/trans isomerase activity and are believed to be involved in protein folding and/or intracellular protein transport. And Cyclophilin A binds to the Gag protein of human immunodeficiency virus type 1 (HIV-1). Additionally, Cyclophilin A may have an essential function in HIV-1 replication.may have an essential function in HIV-1 replication.
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分子量
- 185 kDa
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基因ID
- 5478
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UniProt
- P62937
抗原
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